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Western blot
Immunoprecipitation
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- A302-073A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- hPrp3p Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse hPrp3p by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-hPrp3p (Cat. No. A302-073A Lot2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human hPrp3p by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-hPrp3p (Cat. No. A302-073A Lot2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human hPrp3p by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-hPrp3p antibody A302-073A (lot A302-073A-2) used for IP at 6 µg per reaction. hPrp3p was also immunoprecipitated by a previous lot of this antibody (lot A302-073A-1) and rabbit anti-hPrp3p antibody A302-074A-1. For blotting immunoprecipitated hPrp3p, A302-074A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
