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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- AM26502AF-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti PRPF3
- Antibody type
- Monoclonal
- Antigen
- Human recombinant full-length GSTPrp3
- Reactivity
- Human, Mouse, Rat, Hamster
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 4E3
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Prp3 expression in Jurkat cells (1), HeLa cells (2), Raji cells (3), HL60 cells (4), A431 cells (5), L5178Y cells (6), WR19L cells (7), NIH/3T3 cells (8), CHO cells (9), PC12 cells (10) and Rat1 cells (11) using AM26502AF-N. (The clear band was detected in human cell lines at 116KDa. But the epitope is unclear and is thought that it is the crossreactivity of this monoclonal antibody.)
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunocytochemical detection of Prp3 on HEp-⠡ cells using AM26502AF-N.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunoprecipitation of Prp3 from Jurkat cells with AM26502AF-N (1) or Rat IgG 2a(2) and from L5178Y cells with AM26502AF-N (3) or Rat IgG2a (4). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with AM26502AF-N.
