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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- BS-0776R - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ECM1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Lane 1: MDA-MB-231Cell lysates; Lane 2: MCF-7 Cell lysates probed with ECM1 Polyclonal Antibody, Unconjugated (bs-0776R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed, paraffin embedded Mouse esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ECM1 Polyclonal Antibody, Unconjugated (bs-0776R) at 1:400 overnight at 4°C, followed by a FITC conjugated secondary antibody for 90minutes at 37°C and DAPI for nuclei staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin embedded mouse endometrium tissue labeled with Anti-ECM1 Polyclonal Antibody, Unconjugated (bs-0776R) at 1:300, followed by conjugation to the secondary antibody and DAB staining.
