A305-133A
antibody from Invitrogen Antibodies
Targeting: ZC3H7B
DKFZp434K0920, FLJ13787, KIAA1031, RoXaN
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- A305-133A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZC3H7B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human ZC3H7B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZC3H7B antibody A305-133A (lot A305-133A-1) used for IP at 6 µg per reaction. ZC3H7B was also immunoprecipitated by rabbit anti-ZC3H7B antibody A305-134A. For blotting immunoprecipitated ZC3H7B, A305-133A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse ZC3H7B by western blot. Samples: Whole cell lysate (15 µg) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZC3H7B antibody A305-133A (lot A305-133A-1) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.