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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX110219 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX110219, RRID:AB_10721970
- Product name
- SETDB1 antibody [N2C1], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Sample (30 ?g of whole cell lysate) A: HeLa 5% SDS PAGE GTX110219 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- SETDB1 antibody [N2C1], Internal immunoprecipitates SETDB1 protein in IP experiments.IP samples: HeLa nuclear extractA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of SETDB1 protein by 4 £gg SETDB1 antibody [N2C1], Internal (GTX110219)5 % SDS-PAGEThe immunoprecipitated SETDB1 protein was detected by SETDB1 antibody [N2C1], Internal (GTX110219) diluted at 1:1000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with HeLa chromatin extract and 5 £gg of either control rabbit IgG or anti-SETDB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to p53P2.
