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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
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- Product number
- PAB10044 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10044, RRID:AB_1713853
- Product name
- SETDB1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of SETDB1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly.
mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression.
SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins.
Assignment of a novel bifurcated SET domain gene, SETDB1, to human chromosome band 1q21 by in situ hybridization and radiation hybrids.
Sarraf SA, Stancheva I
Molecular cell 2004 Aug 27;15(4):595-605
Molecular cell 2004 Aug 27;15(4):595-605
mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression.
Wang H, An W, Cao R, Xia L, Erdjument-Bromage H, Chatton B, Tempst P, Roeder RG, Zhang Y
Molecular cell 2003 Aug;12(2):475-87
Molecular cell 2003 Aug;12(2):475-87
SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins.
Schultz DC, Ayyanathan K, Negorev D, Maul GG, Rauscher FJ 3rd
Genes & development 2002 Apr 15;16(8):919-32
Genes & development 2002 Apr 15;16(8):919-32
Assignment of a novel bifurcated SET domain gene, SETDB1, to human chromosome band 1q21 by in situ hybridization and radiation hybrids.
Harte PJ, Wu W, Carrasquillo MM, Matera AG
Cytogenetics and cell genetics 1999;84(1-2):83-6
Cytogenetics and cell genetics 1999;84(1-2):83-6
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- Experimental details
- Western blot analysis is shown using SETDB1 polyclonal antibody (Cat # PAB10044) to detect human SETBD1 present in a 293 whole cell lysate.Approximately 30 ug of lysate was loaded per lane for SDS-PAGE.Comparison to a molecular weight marker (not shown) indicates a singleband of ~170 KDa is detected.Peptide competition (not shown) blocks staining of this band.The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2h followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] diluted1:5,000 for 45 min.IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.
