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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- GTX15824 - Provider product page
- Provider
- GeneTex
- Product name
- PASK antibody [mAB6]
- Antibody type
- Monoclonal
- Reactivity
- Human, Rat, Simian
- Host
- Mouse
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Immunofluorescent analysis of PASK (green) in HeLa cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with PASK antibody [mAB6] in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4¢XC in a humidified chamber. Cells were washed with PBST and incubated with a proper secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
