PA5-29309

antibody from Invitrogen Antibodies

Targeting: PASK KIAA0135, PASKIN, STK37

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29309

Provider

Invitrogen Antibodies

Product name

PASK Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: mouse brain. Predicted reactivity: Mouse (91%), Rat (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Cell type-specific deletion in mice reveals roles for PAS kinase in insulin and glucagon production.

Semplici F, Mondragon A, Macintyre B, Madeyski-Bengston K, Persson-Kry A, Barr S, Ramne A, Marley A, McGinty J, French P, Soedling H, Yokosuka R, Gaitan J, Lang J, Migrenne-Li S, Philippe E, Herrera PL, Magnan C, da Silva Xavier G, Rutter GA
Diabetologia 2016 Sep;59(9):1938-47

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PASK in methanol-fixed HeLa cells using a PASK polyclonal antibody (Product # PA5-29309) at a 1:500 dilution.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 1 Generation of conditional Pask alleles and deletion in beta or alpha cells. ( a ) Knockout (KO) strategy. Generation of beta ( Pask BKO) and alpha ( Pask AKO) cell-specific Pask KO mice by Cre-mediated excision of exons from 12 to 15 encoding the PASK Ser/Thr kinase domain. Human PASK cDNA (modified from [ 22 ]) with the Ser/Thr kinase domain encoding exons circled in purple and enlarged in the black box--a view from the back of PASK kinase domain (amino acids 977-1300) crystal structure (PBD code 3DLS) with the bound ADP molecule in evidence [ 43 ]. ( b ) Mouse Pask gene structure (modified from [ 22 ]) and the location of the loxP sites. ( c ) Example of a genotyping gel indicating the presence of WT and recombined (Cond) alleles. ( d ) Pask and Ins2 gene expression measured by qPCR in Pask BKO (BKO) and control (WT) mouse islets. Immunohistochemical analysis of pancreatic sections from Pask BKO ( e ) and Pask AKO ( f ) mice and controls, with PASK revealed using Alexa 568 (red), and insulin or glucagon revealed using Alexa 488 (green). Insets i-iv are magnified areas, as shown in the main images. Insets v-viii are example images of no-primary-antibody controls for each of the genotypes, with insets v and vii showing the DAPI staining for nuclei; scale bars, 100 mum. PASK fluorescence was displayed in 87.6 +- 1.5% of insulin-positive cells in WT islets vs 10.5 +- 2.3% in Pask BKO islets; n = 7 mice per genotype for both Pask BKO and Pask AKO mice; * p < 0.05 and ** p