14899-1-AP

antibody from Proteintech Group

Targeting: PSMD4 AF, AF-1, Rpn10, S5A

Provider product page for 14899-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [14]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 14899-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#14899-1-AP, RRID:AB_2170627
Product name: PSMD4 antibody
Antibody type: Polyclonal
Description: KD/KO validated PSMD4 antibody (Cat. #14899-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

PSMD4 protein structure - 14899-1-AP shown in red.

Submitted references Molecular basis of SAP05-mediated ubiquitin-independent proteasomal degradation of transcription factors.

Yan X, Yuan X, Lv J, Zhang B, Huang Y, Li Q, Ma J, Li Y, Wang X, Li Y, Yu Y, Liu Q, Liu T, Mi W, Dong C
Nature communications 2024 Feb 8;15(1):1170

PSMD4 drives progression of hepatocellular carcinoma via Akt/COX2 pathway and p53 inhibition.

Zhang J, Fang S, Rong F, Jia M, Wang Y, Cui H, Hao P
Human cell 2023 Sep;36(5):1755-1772

Ubiquitin receptors play redundant roles in the proteasomal degradation of the p53 repressor MDM2.

Sparks A, Kelly CJ, Saville MK
FEBS letters 2022 Nov;596(21):2746-2767

Proteasome inhibition by bortezomib parallels a reduction in head and neck cancer cells growth, and an increase in tumor-infiltrating immune cells.

Benvenuto M, Ciuffa S, Focaccetti C, Sbardella D, Fazi S, Scimeca M, Tundo GR, Barillari G, Segni M, Bonanno E, Manzari V, Modesti A, Masuelli L, Coletta M, Bei R
Scientific reports 2021 Sep 24;11(1):19051

Generation and identification of a conditional knockout allele for the PSMD11 gene in mice.

Zhao L, Zhao J, Zhang Y, Wang L, Zuo L, Niu A, Zhang W, Xue X, Zhao S, Sun C, Li K, Wang J, Bian Z, Zhao X, Saur D, Seidler B, Wang C, Qi T
BMC developmental biology 2021 Feb 1;21(1):4

Proteaphagy in Mammalian Cells Can Function Independent of ATG5/ATG7.

Goebel T, Mausbach S, Tuermer A, Eltahir H, Winter D, Gieselmann V, Thelen M
Molecular & cellular proteomics : MCP 2020 Jul;19(7):1120-1131

Defective proteasome biogenesis into skin fibroblasts isolated from Rett syndrome subjects with MeCP2 non-sense mutations.

Sbardella D, Tundo GR, Cunsolo V, Grasso G, Cascella R, Caputo V, Santoro AM, Milardi D, Pecorelli A, Ciaccio C, Di Pierro D, Leoncini S, Campagnolo L, Pironi V, Oddone F, Manni P, Foti S, Giardina E, De Felice C, Hayek J, Curatolo P, Galasso C, Valacchi G, Coletta M, Graziani G, Marini S
Biochimica et biophysica acta. Molecular basis of disease 2020 Jul 1;1866(7):165793

Loss of nuclear UBE3A causes electrophysiological and behavioral deficits in mice and is associated with Angelman syndrome.

Avagliano Trezza R, Sonzogni M, Bossuyt SNV, Zampeta FI, Punt AM, van den Berg M, Rotaru DC, Koene LMC, Munshi ST, Stedehouder J, Kros JM, Williams M, Heussler H, de Vrij FMS, Mientjes EJ, van Woerden GM, Kushner SA, Distel B, Elgersma Y
Nature neuroscience 2019 Aug;22(8):1235-1247

The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner.

Liu X, Chen L, Ge J, Yan C, Huang Z, Hu J, Wen C, Li M, Huang D, Qiu Y, Hao H, Yuan R, Lei J, Yu X, Shao J
Cancer research 2016 Aug 15;76(16):4897-907

The transition zone protein Rpgrip1l regulates proteasomal activity at the primary cilium.

Gerhardt C, Lier JM, Burmühl S, Struchtrup A, Deutschmann K, Vetter M, Leu T, Reeg S, Grune T, Rüther U
The Journal of cell biology 2015 Jul 6;210(1):115-33

Chaperone-mediated 26S proteasome remodeling facilitates free K63 ubiquitin chain production and aggresome clearance.

Nanduri P, Hao R, Fitzpatrick T, Yao TP
The Journal of biological chemistry 2015 Apr 10;290(15):9455-64

The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.

Sparks A, Dayal S, Das J, Robertson P, Menendez S, Saville MK
Oncogene 2014 Sep 18;33(38):4685-96

S5a binds to death receptor-6 to induce THP-1 monocytes to differentiate through the activation of the NF-κB pathway.

Wang Z, Fan C, Zhou HF, Lu JS, Sun MJ, Song JW, Le Y, Jiang HD, Wang LH, Jiao BH
Journal of cell science 2014 Aug 1;127(Pt 15):3257-68

Interaction of HPV E6 oncoproteins with specific proteasomal subunits.

Tomaić V, Ganti K, Pim D, Bauer C, Blattner C, Banks L
Virology 2013 Nov;446(1-2):389-96

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: K-562 cells were subjected to SDS PAGE followed by western blot with 14899-1-AP(PSMD4 antibody) at dilution of 1:3000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using PSMD4 antibody 14899-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line