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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AF6067 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat BOK Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human, mouse, and rat BOK in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9UMX3- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells.
Li X, Lu J, Kan Q, Li X, Fan Q, Li Y, Huang R, Slipicevic A, Dong HP, Eide L, Wang J, Zhang H, Berge V, Goscinski MA, Kvalheim G, Nesland JM, Suo Z
Scientific reports 2017 Jul 11;7(1):5081
Scientific reports 2017 Jul 11;7(1):5081
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Human, Mouse, and Rat BOK by Western Blot. Western blot shows lysates of PC-3 human prostate cancer cell line, Hepa 1-6 mouse hepatoma cell line, and rat testis tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat BOK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6067) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BOK at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
