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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
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- Product number
- LS-C669059 - Provider product page
- Provider
- LSBio
- Product name
- PDCD1 / CD279 / PD-1 Antibody (clone 8A4) LS-C669059
- Antibody type
- Monoclonal
- Description
- Protein A purified
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 8A4
- Storage
- Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of PD-1 in transfected 293 cell lysate with PD-1 antibody at (A) 0.5 and (B) 1 ug/mL.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Titration curve analysis of PD-1 mAbs to detect recombinant PD-1 in ELISA with RF16001, RF16002, RF16003, RF16004, and RF16005 abs at decreasing concentrations.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunocytochemistry of PD-1 in transfected 293 cells with PD-1 antibody at 5 ug/mL. Lower left: Immunocytochemistry in transfected 293 cells with control mouse IgG antibody at 5 ug/mL.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunocytochemistry of PD-1 in transfected 293 cells with PD-1 antibody at 5 ug/mL. Lower left: Immunocytochemistry in transfected 293 cells with control mouse IgG antibody at 5 ug/mL.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunocytochemistry of PD-1 in transfected 293 cells with PD-1 antibody at 5 ug/mL. Lower left: Immunocytochemistry in transfected 293 cells with control mouse IgG antibody at 5 ug/mL.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of PD-1 in (A) human breast cancer tissue and (B) human normal breast tissue with PD-1 antibody at 5 ug/mL.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of PD-1 in (A) human tonsil tissue, (B) human lymph node tissue, and (C) human spleen tissue with PD-1 antibody at 5 ug/mL. (D) Immunohistochemistry in human tonsil tissue with control mouse IgG staining at 5 ug/mL.
