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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- TA806811S - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDCD1 Monoclonal Antibody (OTI11B2), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI11B2
- Vial size
- 30 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1. Positive lysates LY401555 (100 µg) and LC401555 (20 µg) can be purchased separately from OriGene.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA806811 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA806811)(Left). The right is negative control. (1:100)(1:900)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human tonsil using anti-PDCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806811)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of stable expression PD1 cells using anti-PDCD1 antibody (TA806811) (Red) compared to a nonspecific negative control antibody (Blue). (1:50)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either mouse PD1(MR227347) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDCD1 antibody (TA806811),compared to a nonspecific negative control antibody (green),and then analyzed by flow cytometry. (1:50)
