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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-21330 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- S100A11 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A431. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Regulated cell death joins in atherosclerotic plaque silent progression.
Uyy E, Suica VI, Boteanu RM, Cerveanu-Hogas A, Ivan L, Hansen R, Antohe F
Scientific reports 2022 Feb 18;12(1):2814
Scientific reports 2022 Feb 18;12(1):2814
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of S100A11 using 30 µg A431 whole cell lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with a S100A11 polyclonal antibody (Product # PA5-21330) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of S100A11 in paraformaldehyde-fixed HeLa cells using a S100A11 polyclonal antibody (Product # PA5-21330) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using S100A11 antibody (Product # PA5-21330) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using S100A11 (Product # PA5-21330) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Validation of mass spectrometry data by Western blot and quantitative qPCR methods. The Western blot immune detection and associated densitometry for selected protein markers: ( a ) VDAC3, ( b ) CALR and ( c ) S100A11 and mRNA gene expression level for: ( d ) VDAC3, ( e ) CALR, ( f ) FTL, ( g ) TF, ( h ) TLR2 and ( i ) TLR4 in aortic homogenate prepared from C, Ae, As and Al groups normalized to beta actin protein and gene expression, respectively. C: Control group, Ae: early atherosclerotic group, As: statin and PCSK9 antibody stabilized atherosclerotic group and Al: late atherosclerotic group. Protein and gene levels found for the selected markers are in good corroboration with the mass spectrometry results. Statistical analysis was performed using t Student's test and results show mean +- SD (* p < 0.05; ** p < 0.01 and *** p < 0.001; n > 4). VDAC3: voltage-dependent anion-selective channel protein three, CALR: calreticulin, S100A11: protein S100A11, FTL: ferritin light chain, TF: transferrin, TLR2: toll like receptor 2 and TLR4: toll like receptor 4. The original blots images of the membranes from this figure can be found in Supplementary S1 .
