PAB20727

antibody from Abnova Corporation

Targeting: KLHDC8B MGC35097

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20727

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20727, RRID:AB_10966238

Product name

KLHDC8B polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant KLHDC8B.

Antigen sequence

MPTPCYGASTFLHGNKIYVLGGRQGKLPVTAFEAF
DLEARTWTRHPSLPSRRAFAGCAMAEGSVFSLGGL
QQPGPHNFYSRPHFVNTVEMFDLEHGSWTKLPRSL
RMRDKRADFVVGSL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of cell lysates with KLHDC8B polyclonal antibody (Cat # PAB20727) at 1:250-1:500 dilution.Lane 1 : NIH/3T3Lane 2 : NBT-II

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with KLHDC8B polyclonal antibody (Cat # PAB20727) at 1-4 ug/mL dilution shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex with KLHDC8B polyclonal antibody (Cat # PAB20727) shows strong cytoplasmic positivity in glial cells (astrocytes) at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)