AZT-001-25UL

antibody from Invitrogen Antibodies

Targeting: SLC39A1 ZIP1, ZIRTL

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AZT-001-25UL

Provider

Invitrogen Antibodies

Product name

ZIP1 (SLC39A1) (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

25 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human U-87 MG glioblastoma cell lysate (lanes 1 and 3) and human MDA-231 breast adenocarcinoma cell lysate (lanes 2 and 4): - 1,2. Anti-ZIP1 (SLC39A1) (extracellular) Antibody (#AZT-001), (1:200).3,4. Anti-ZIP1 (SLC39A1) (extracellular) Antibody , preincubated with ZIP1/SLC39A1 (extracellular) Blocking Peptide (#BLP-ZT001).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4)brain lysates: - 1,2. Anti-ZIP1 (SLC39A1) (extracellular) Antibody (#AZT-001), (1:200).3,4. Anti-ZIP1 (SLC39A1) (extracellular) Antibody , preincubated with ZIP1/SLC39A1 (extracellular) Blocking Peptide (#BLP-ZT001).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human U-87 MG glioblastoma cell lysate (lanes 1 and 3) and human MDA-231 breast adenocarcinoma cell lysate (lanes 2 and 4): - 1,2. Anti-ZIP1 (SLC39A1) (extracellular) Antibody (#AZT-001), (1:200).3,4. Anti-ZIP1 (SLC39A1) (extracellular) Antibody , preincubated with ZIP1/SLC39A1 (extracellular) Blocking Peptide (#BLP-ZT001).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of ZIP1 in rat hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-ZIP1 (SLC39A1) (extracellular) Antibody (#AZT-001), (1:400), followed by Anti-rabbit-Alexa-488 Antibody . ZIP1 is expressed (green) in the mossy fiber (MF) terminal field (arrows) of the CA3 hippocampal region. Nuclei are labeled with DAPI (blue).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cell surface detection of ZIP1 (SLC39A1) by indirect flow cytometry in live intact human K562 chronic myelogenous leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-ZIP1 (SLC39A1) (extracellular) Antibody (#AZT-001), (5μg) + goat- Anti-rabbit-FITC.