- MA1-80456 - Invitrogen Antibodies IL6R antibody

Submitted references IL11 Activates Pancreatic Stellate Cells and Causes Pancreatic Inflammation, Fibrosis and Atrophy in a Mouse Model of Pancreatitis.

Ng B, Viswanathan S, Widjaja AA, Lim WW, Shekeran SG, Goh JWT, Tan J, Kuthubudeen F, Lim SY, Xie C, Schafer S, Adami E, Cook SA
International journal of molecular sciences 2022 Mar 24;23(7)

Hepatocyte-specific IL11 cis-signaling drives lipotoxicity and underlies the transition from NAFLD to NASH.

Dong J, Viswanathan S, Adami E, Singh BK, Chothani SP, Ng B, Lim WW, Zhou J, Tripathi M, Ko NSJ, Shekeran SG, Tan J, Lim SY, Wang M, Lio PM, Yen PM, Schafer S, Cook SA, Widjaja AA
Nature communications 2021 Jan 4;12(1):66

Involvement of hepcidin in the anemia of multiple myeloma.

Sharma S, Nemeth E, Chen YH, Goodnough J, Huston A, Roodman GD, Ganz T, Lichtenstein A
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Jun 1;14(11):3262-7

Interferon alpha induces cell death through interference with interleukin 6 signaling and inhibition of STAT3 activity.

Thyrell L, Arulampalam V, Hjortsberg L, Farnebo M, Grandér D, Pokrovskaja Tamm K
Experimental cell research 2007 Nov 15;313(19):4015-24

Retinoic acid inhibits IL-6-dependent but not constitutive STAT3 activation in Epstein-Barr virus-immortalized B lymphocytes.

Zancai P, Cariati R, Quaia M, Guidoboni M, Rizzo S, Boiocchi M, Dolcetti R
International journal of oncology 2004 Aug;25(2):345-55

Interleukin-6 enhances whereas tumor necrosis factor alpha and interferons inhibit integrin expression and adhesion of human mast cells to extracellular matrix proteins.

Schoeler D, Grützkau A, Henz BM, Küchler J, Krüger-Krasagakis S
The Journal of investigative dermatology 2003 May;120(5):795-801

Human herpesvirus 8-encoded interleukin 6 activates HIV-1 in the U1 monocytic cell line.

Gage JR, Breen EC, Echeverri A, Magpantay L, Kishimoto T, Miles S, Martínez-Maza O
AIDS (London, England) 1999 Oct 1;13(14):1851-5

Human herpesvirus 8-encoded interleukin 6 activates HIV-1 in the U1 monocytic cell line.

Gage JR, Breen EC, Echeverri A, Magpantay L, Kishimoto T, Miles S, Martínez-Maza O
AIDS (London, England) 1999 Oct 1;13(14):1851-5

Selective proteolytic cleavage of IL-2 receptor and IL-6 receptor ligand binding chains by neutrophil-derived serine proteases at foci of inflammation.

Bank U, Reinhold D, Schneemilch C, Kunz D, Synowitz HJ, Ansorge S
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 1999 Nov;19(11):1277-87

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 1 IL11RA is highly expressed in hepatocytes and IL11 cis -signaling is hepatotoxic. a Immunohistochemistry staining of IL11RA and IL6R in healthy human liver sections (scale bars, 20 um, n = 1 independent experiment, due to limited amount of human liver section). b Flow cytometry forward scatter (FSC) plots of IL11RA, IL6R, and gp130 staining and fluorescence intensity plots of IL11RA and IL6R staining on hepatocytes and THP-1. c Abundance of IL11RA1 and IL6R reads in hepatocytes at baseline based on RNA-seq (left) and Ribo-seq (right) (transcripts per million, TPM) ( n = 3). d , e Read coverage of d IL11RA1 and e IL6R transcripts based on RNA-seq (gray) and Ribo-seq (red) of primary human hepatocytes ( n = 3). f Western blots showing ERK, JNK, and STAT3 activation status and g ALT secretion ( n = 4) by hepatocytes following a dose range stimulation of either hyperIL11 or hyperIL6. h ALT levels in the supernatants of hepatocytes stimulated with hyperIL11 alone or in the presence of increasing amounts of soluble gp130 (sgp130) ( n = 4). i , j Western blots of hepatocyte lysates showing i phosphorylated ERK and JNK and their respective total expression in response to hyperIL11 stimulation alone or with sgp130 and j phospho-STAT3 and total STAT3 in response to hyperIL6 stimulation with and without sgp130. k Representative FSC plots of propidium Iodide (PI) staining of IL11-stimulated hepatocytes in the presence of sgp130 or soluble IL11RA (sIL11RA). l Western blots showing

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 IL11 induces pancreatic stellate cell activation and invasion. ( a ) t-distributed stochastic neighbour embedding (tSNE) plots showing scRNA-seq expression of Il11ra1 , Il6ra and Il-6st ( gp130 ) in mouse pancreatic tissue. Cell clusters were identified using the Tabula Muris web tool ( https://tabula-muris.ds.czbiohub.org/ (accessed on 21 February 2022)). []. Black arrows indicate pancreatic stellate cells (PSCs). ( b ) Representative immunostaining images of gp130, IL11RA, IL6RA in PSCs. Cells were counterstained with DAPI. Scale bars: 50 um. ( c ) Western blot analysis of phosphorylated and total ERK and STAT3 and alphaSMA in lysates from PSC treated with IL11 (10 ng/mL) or IL 6 (10 ng/mL) across the indicated time-points (0 to 24 h). GAPDH serves as a loading control. ( d ) ELISA-based quantification of secreted MMP2 levels in PSC supernatants. ( e , f ) Representative immunofluorescence images and quantification of alphaSMA +ve cells, Collagen I intensity/area and EdU +ve cells at baseline and after 24 h treatment with either recombinant human TGFbeta1 (5 ng/mL), IL11 (5 ng/mL), bFGF (10 ng/mL), CTGF (50 ng/mL), PDGF (200 ng/mL) or EDN1 (250 ng/mL). Cells were counterstained with DAPI. Scale bar: 200 um. ( g ) Matrigel invasion capacity of PSCs was determined at baseline and after 24 h treatment with PDGF (20 ng/mL) or with increasing concentrations of IL11 (5-20 ng/mL). Scale bars: 150 um. AU: Arbitrary Unit. Data are represented as mean +- SD in panel ( d ) an

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