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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- FAB227A-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-6R alpha AP-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-6 R alpha in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity or interference was observed with recombinant human (rh) IL-1 alpha , recombinant mouse (rm) IL-1 alpha , rhIL-1 beta , rmIL-1 beta , rhIL-1ra, rhIL-2, rhIL-3, rmIL-3, rhIL-4, rmIL-4, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL-10, or rhIL-11.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Antigen sequence
P08887- Isotype
- IgG
- Antibody clone number
- 17506
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Immunophenotypic characterization of human monocyte subsets: possible implications for cardiovascular disease pathophysiology.
Shantsila E, Wrigley B, Tapp L, Apostolakis S, Montoro-Garcia S, Drayson MT, Lip GY
Journal of thrombosis and haemostasis : JTH 2011 May;9(5):1056-66
Journal of thrombosis and haemostasis : JTH 2011 May;9(5):1056-66
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of IL-6 R alpha in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human IL-6 R alpha APC-conjugated Monoclonal Antibody (Catalog # FAB227A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
