BMS135

antibody from Invitrogen Antibodies

Targeting: IL6R CD126

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

BMS135

Provider

Invitrogen Antibodies

Product name

CD126 Monoclonal Antibody (B-R6), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: Recognizes soluble and membranous human IL-6R. This Antibody has been shown to inhibit IL-6 mediated proliferation of XG-1 cells, and partially blocks binding of IL-6 to its receptor. BMS135 is applicable for FACS analysis IL-6 is a multifunctional cytokine involved in the regulation of the immune response, hematopoiesis and acute phase response. It has been recognized to be a member of the alpha-helical cytokine family. IL-6 exerts its action via a cell surface receptor which consists of two subunits, an 80 kDa ligand binding subunit (gp80) of 468 amino acids and a 130 kDa signal transducing protein (gp130) of 896 amino acid residues. Characterisation of the extracellular portion of the 80 kDa IL-6 receptor revealed the existence of a single immunoglobulin-like domain in the NH2-terminal of the extracellular region, which does not contribute to ligand binding. The remainder of the extracellular domain however is essential for low affinity ligand binding, which consecutively triggers the association of the receptor and gp130 thus forming a high affinity binding site for IL-6. A soluble form of the human gp80 protein has been detected in serum and urine samples. This 55 kDa protein representing the extracellular portion of gp80 is generated by shedding, a process that seems to be controlled by protein kinase C. It is still functional, indicating that soluble gp80 plays a biological role in promoting IL-6 activity. So far, the soluble IL-6 receptor is unique in acting as an agonist together with its ligand. Applications Tested: Flow Cytometry, Functional Studies (Blocking). Purity: >95%.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

B-R6

Vial size

100 µg

Concentration

0.1 mg/mL

Storage

4° C

References

Submitted references

Secreted KIAA1199 promotes the progression of rheumatoid arthritis by mediating hyaluronic acid degradation in an ANXA1-dependent manner.

Zhang W, Yin G, Zhao H, Ling H, Xie Z, Xiao C, Chen Y, Lin Y, Jiang T, Jin S, Wang J, Yang X
Cell death & disease 2021 Jan 20;12(1):102

---

Crohn's Disease Fibroblasts Overproduce the Novel Protein KIAA1199 to Create Proinflammatory Hyaluronan Fragments.

Soroosh A, Albeiroti S, West GA, Willard B, Fiocchi C, de la Motte CA
Cellular and molecular gastroenterology and hepatology 2016 May;2(3):358-368.e4

---

A new strategy to deliver synthetic protein drugs: self-reproducible biologics using minicircles.

Yi H, Kim Y, Kim J, Jung H, Rim YA, Jung SM, Park SH, Ju JH
Scientific reports 2014 Aug 5;4:5961

---

Legionella pneumophila type II secretion dampens the cytokine response of infected macrophages and epithelia.

McCoy-Simandle K, Stewart CR, Dao J, DebRoy S, Rossier O, Bryce PJ, Cianciotto NP
Infection and immunity 2011 May;79(5):1984-97

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 6 IL-6 promotes KIAA1199 expression through the PI3K/Akt/NF-kappaB signaling pathway in RA FLS. A - D Relative mRNA expression of KIAA1199 in FLS from healthy subjects ( n = 9) stimulated without (Control) or with 1 and 5 ng/ml TNF-alpha ( A ), IL-1beta ( B ), IL-6 ( C ), and TGF-beta1 ( D ), respectively. E - G Inhibitory effect of IL-6R blocking mAb on the expression of KIAA1199 mRNA ( E ), protein ( F ) in FLS from RA patients ( n = 9), and the formation of LMW-HA in the medium ( G ). H Western blot analysis of cytoplasmic PI3K, p-Akt, toatl-Akt, p-STAT3, total-STAT3, and intranuclear p-P65 in RA FLS stimulated with 5 ng/ml IL-6 at 0, 15, 30 min. I Effect of ly294002, GSK690693, and Stattic on the mRNA expression of KIAA1199 in response to 1 ng/ml IL-6. J , K Effect of siRNA knockdown of PI3K ( J ) and STAT3 ( K ) on the protein expression of KIAA1199 in RA FLS stimulated without or with 1 ng/ml IL-6. All reactions were conducted in triplicate and the data were presented with mean +- SD.