Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AB-227-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-6R alpha Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects human IL-6R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse IL-6R alpha and recombinant human LIF is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P08887
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Lipopolysaccharide-squamous cell carcinoma-monocyte interactions induce cancer-supporting factors leading to rapid STAT3 activation.
Kurago ZB, Lam-ubol A, Stetsenko A, De La Mater C, Chen Y, Dawson DV
Head and neck pathology 2008 Mar;2(1):1-12
Head and neck pathology 2008 Mar;2(1):1-12
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IL-6 R alpha Enhancement of IL-6-dependent Inhibition of Cell Proliferation and Neutralization by Human IL-6 R alpha Antibody. Recombinant Human IL-6 R alpha (Catalog # 227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant Human IL-6 R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-6 R alpha Polyclonal Antibody (Catalog # AB-227-NA). The ND50 is typically 1-4 µg/mL.