730048

antibody from Invitrogen Antibodies

Targeting: L3MBTL2 dJ756G23.3, DKFZP761I141, H-l(3)mbt-l

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

730048

Provider

Invitrogen Antibodies

Product name

Anti-L3MBTL2 Monoclonal Antibody, Biotin

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Mouse

Conjugate

Biotin

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

References

Submitted references

Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation.

Marcon E, Jain H, Bhattacharya A, Guo H, Phanse S, Pu S, Byram G, Collins BC, Dowdell E, Fenner M, Guo X, Hutchinson A, Kennedy JJ, Krastins B, Larsen B, Lin ZY, Lopez MF, Loppnau P, Miersch S, Nguyen T, Olsen JB, Paduch M, Ravichandran M, Seitova A, Vadali G, Vogelsang MS, Whiteaker JR, Zhong G, Zhong N, Zhao L, Aebersold R, Arrowsmith CH, Emili A, Frappier L, Gingras AC, Gstaiger M, Paulovich AG, Koide S, Kossiakoff AA, Sidhu SS, Wodak SJ, Gräslund S, Greenblatt JF, Edwards AM
Nature methods 2015 Aug;12(8):725-31

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis using Anti-L3MBTL2 Recombinant Mouse Monoclonal Antibody (Product # 730048) followed by western blot detection. Immunoprecipitation was performed on HEK293 cells over expressing flag-tagged L3MBTL2 protein. Western blot using an anti-flag antibody was performed on the raw lysate (lane 1) and immunoprecipitated protein (lane 2) to evaluate the results of Immunoprecipitation. Because detection utilizes an anti-flag antibody, the presence of multiple bands suggests multiple forms of flag-tagged L3MBTL2 protein rather than cross-reactivity of the Anti-L3MBTL2 Recombinant Mouse Monoclonal Antibody.

Conjugate

Biotin