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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- NBP1-32621 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-32621, RRID:AB_2133212
- Product name
- Rabbit Polyclonal L3MBTL2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: L3MBTL2 Antibody [NBP1-32621] - Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of L3MBTL2 protein by 2. 5 ug of L3MBTL2 antibody 5% SDS-PAGE gel.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: L3MBTL2 Antibody [NBP1-32621] - (30 ug whole cell lysate) A:MOLT4 7.5% SDS PAGE diluted at 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: L3MBTL2 Antibody [NBP1-32621] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with L3MBTL2 antibody diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: L3MBTL2 Antibody [NBP1-32621] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with L3MBTL2 antibody diluted at 1:500.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: L3MBTL2 Antibody [NBP1-32621] - Sample: HeLa whole cell lysate/extract A. 5 ug preimmune rabbit IgG, B. 5 ug of L3MBTL2 antibody The precipitated DNA was detected by PCR with primer set targeting to CDC7 gene locus.
