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antibody from Invitrogen Antibodies
Targeting: L3MBTL2
dJ756G23.3, DKFZP761I141, H-l(3)mbt-l
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Chromatin Immunoprecipitation [1]
- Other assay [2]
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- Product number
- PA5-28549 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- L3MBTL2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, mouse testis. Predicted reactivity: Mouse (88%), Rat (87%), Bovine (91%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Genome-wide CRISPR screen identifies suppressors of endoplasmic reticulum stress-induced apoptosis.
Panganiban RA, Park HR, Sun M, Shumyatcher M, Himes BE, Lu Q
Proceedings of the National Academy of Sciences of the United States of America 2019 Jul 2;116(27):13384-13393
Proceedings of the National Academy of Sciences of the United States of America 2019 Jul 2;116(27):13384-13393
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of L3MBTL2 was performed by separating 50 µg of Mouse tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a L3MBTL2 Polyclonal Antibody (Product # PA5-28549) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of L3MBTL2 was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a L3MBTL2 Polyclonal Antibody (Product # PA5-28549) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- L3MBTL2 antibody immunoprecipitates L3MBTL2 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract. A: 5 µg preimmune rabbit IgG. B: 5 µg of L3MBTL2 antibody (Product # PA5-28549). The precipitated DNA was detected by PCR with primer set targeting to CDC7 gene locus.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- L3MBTL2 Polyclonal Antibody immunoprecipitates L3MBTL2 protein in IP experiments. IP Sample: 1,000 µg HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2.5 µg of preimmune rabbit IgG C. Immunoprecipitation of L3MBTL2 protein by 2.5 µg of L3MBTL2 Polyclonal Antibody (Product # PA5-28549) 5% SDS-PAGE The immunoprecipitated L3MBTL2 protein was detected by L3MBTL2 Polyclonal Antibody (Product # PA5-28549) diluted at 1:1,000.
