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- Antibody Data
- Antigen structure
- References [3]
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- Western blot [1]
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- Product number
- PAB10261 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10261, RRID:AB_1676429
- Product name
- MFAP5 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MFAP5.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The extracellular matrix protein MAGP-2 interacts with Jagged1 and induces its shedding from the cell surface.
The exon structure of the human MAGP-2 gene. Similarity with the MAGP-1 gene is confined to two exons encoding a cysteine-rich region.
Further characterization of proteins associated with elastic fiber microfibrils including the molecular cloning of MAGP-2 (MP25)
Nehring LC, Miyamoto A, Hein PW, Weinmaster G, Shipley JM
The Journal of biological chemistry 2005 May 27;280(21):20349-55
The Journal of biological chemistry 2005 May 27;280(21):20349-55
The exon structure of the human MAGP-2 gene. Similarity with the MAGP-1 gene is confined to two exons encoding a cysteine-rich region.
Hatzinikolas G, Gibson MA
The Journal of biological chemistry 1998 Nov 6;273(45):29309-14
The Journal of biological chemistry 1998 Nov 6;273(45):29309-14
Further characterization of proteins associated with elastic fiber microfibrils including the molecular cloning of MAGP-2 (MP25)
Gibson MA, Hatzinikolas G, Kumaratilake JS, Sandberg LB, Nicholl JK, Sutherland GR, Cleary EG
The Journal of biological chemistry 1996 Jan 12;271(2):1096-103
The Journal of biological chemistry 1996 Jan 12;271(2):1096-103
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using MFAP5 polyclonal antibody (Cat # PAB10261) shows detection (arrowhead) of secreted MFAP5 (lane 2) and MFAP5 present in a MFAP5 transfected HEK293 lysate (lane 3).No staining is detected in supernatants from non-transfected cells (lane 1).After SDS-PAGE and transfer the membrane was probed with the primary antibody diluted to 1:100 in TBST with 5% BSA overnight at 4°C.Personal Communication, Micheal Birrer, CCR-NCI, Bethesda, MD.
