Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NBP1-82803 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-82803, RRID:AB_11034173
- Product name
- Rabbit Polyclonal MAGP-2/MFAP5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human MAGP-2/MFAP5 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Calcium-dependent FAK/CREB/TNNC1 signalling mediates the effect of stromal MFAP5 on ovarian cancer metastatic potential.
Leung CS, Yeung TL, Yip KP, Pradeep S, Balasubramanian L, Liu J, Wong KK, Mangala LS, Armaiz-Pena GN, Lopez-Berestein G, Sood AK, Birrer MJ, Mok SC
Nature communications 2014 Oct 3;5:5092
Nature communications 2014 Oct 3;5:5092
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MAGP-2/MFAP5 Antibody [NBP1-82803] - Analysis in control (vector only transfected HEK293T lysate) and MFAP5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MAGP-2/MFAP5 Antibody [NBP1-82803] - Analysis in human cell line BEWO.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803] - Staining of human cerebral cortex shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803] - Staining of human smooth muscle shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803] - Staining in human smooth muscle and cerebral cortex tissues using anti-MFAP5 antibody. Corresponding MFAP5 RNA-seq data are presented for the same tissues.