PA5-41014

antibody from Invitrogen Antibodies

Targeting: KCNN3 hSK3, KCa2.3, SKCA3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-41014

Provider

Invitrogen Antibodies

Product name

KCNN3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide sequence: ITELNDRSED LEKQIGSLES KLEHLTASFN SLPLLIADTL RQQQQQLLSA

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of mouse kidney, whole brain lysate using an anti-KCNN3 polyclonal antibody (Product # PA5-41014).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-KCNN3 Polyclonal Antibody (Product # PA5-41014) and a 90 kDa band corresponding to KCNN3 was observed in the Mouse and Rat Brain tissues where as no band was observed in Cerebellum and Spleen which are very low expression models for KCNN3. Tissue extracts (100 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Cerebellum (Lane 3), Mouse Spleen (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:10000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Atto Ultimate Sensitivity Substrate (Product # A38556). * indicates a non specific band observed in Rat Brain.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human daudi cell lysate using an anti-KCNN3 polyclonal antibody (Product # PA5-41014).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (paraffin-embedded) analysis of Rhesus macaque spinal cord cells using an anti-KCNN3 polyclonal antibody (Product # PA5-41014). Primary Antibody Dilution:1:300; Secondary Antibody: Donkey anti Rabbit 488; Secondary Antibody Dilution: 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (paraffin-embedded) analysis of human adult heart tissue using an anti-KCNN3 polyclonal antibody (Product # PA5-41014).