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- Antibody Data
- Antigen structure
- References [1]
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- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-24240 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PYGO2 Monoclonal Antibody (539730)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, no cross-reactivity with recombinant human Pygopus-1 or recombinant mouse Pygopus-1 is observed. Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 539730
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Discovery of an Orally Bioavailable Small-Molecule Inhibitor for the β-Catenin/B-Cell Lymphoma 9 Protein-Protein Interaction.
Wang Z, Zhang M, Quereda V, Frydman SM, Ming Q, Luca VC, Duckett DR, Ji H
Journal of medicinal chemistry 2021 Aug 26;64(16):12109-12131
Journal of medicinal chemistry 2021 Aug 26;64(16):12109-12131
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 0.5 µg/mL of mouse Anti-human Pygopus-2 Monoclonal Antibody (Product # MA5-24240) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for Pygopus-2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 0.5 µg/mL of mouse Anti-human Pygopus-2 Monoclonal Antibody (Product # MA5-24240) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for Pygopus-2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PYGO2 in HeLa human cervical epithelial carcinoma cell line, MCF‚7 human breast cancer cell line, and Daudi human Burkitts lymphoma cell line. Samples were incubated in PYGO2 monoclonal antibody (Product # MA5-24240) using a dilution of 0.5 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for Pygopus‚2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PYGO2 in 0.2 mg/mL lysates of HeLa human cervical epithelial carcinoma cell line. Samples were incubated in PYGO2 monoclonal antibody (Product # MA5-24240) using a dilution of 5 µg/mL. A specific band was detected for Pygopus‚2 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of PYGO2 in immersion fixed MCF‚7 human breast cancer cell line. Samples were incubated in PYGO2 monoclonal antibody (Product # MA5-24240) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PYGO2 in immersion fixed paraffin-embedded sections of normal human ovary (left panel) and ovarian cancer tissue (right panel). Samples were incubated in PYGO2 monoclonal antibody (Product # MA5-24240) using a dilution of 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei.
