Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX79230 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX79230, RRID:AB_11162739
- Product name
- HCN3 antibody [TLL6C5]
- Antibody type
- Monoclonal
- Host
- Rat
- Vial size
- 100 µl
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of TCR V alpha 12.1 in 25 ug of Jurkat, CEM and rat thymus lysates. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with TCR V alpha 12.1 antibody [6D6.6] at a dilution of 1:10 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody. Chemiluminescent detection was performed.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometry analysis of TCR V alpha 12.1 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with TCR V alpha 12.1 antibody [6D6.6] at a dilution of 2 μg/test for 60 min at room temperature. Cells were re-suspended in PBS for FACS analysis.
