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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-111879 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HCN3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- eFluor® 506 can be excited with the violet laser line (405 nm) and emits at 506 nm. We recommend using a 510/20 band pass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HCN3 was performed in rat brain lysate. Lane 1: HCN3 Antibody (Product # PA5-111879) at a dilution of 1:200. Lane 2: HCN3 Antibody, preincubated with the control peptide antigen.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HCN3 in frozen rat dorsal root ganglia (DRG) tissue sections using HCN3 Antibody (Product # PA5-111879) at a dilution of 1:50 followed by mouse anti-rabbit Alexa Fluor 555 (red). Staining is present in neuronal cell bodies. Hoechst 33342 (blue) is used as counterstain.
