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Western blot
Immunohistochemistry
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Other assay [1]
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- Product number
- MA3-902 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HCN3 Monoclonal Antibody (TLL6C5)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA3-902 detects HCN3 from human, mouse and rat samples. MA3-902 has been successfully used in Western blotting and immunohistochemistry procedures. By Western blot MA3-902 detects a ~90 KDa band representing HCN3. The MA3-902 immunogen is a synthetic peptide corresponding to residues T(640) L L A R S A R R S A G S P A S P L V P V R A G P L L A R G P W A S T S(675) of rat HCN3. MA3-902 (clone TLL6C5) is a rat monoclonal hybridoma, produced by immunizing a rat, isolating the spleen cells, creating the hybridoma and successively injecting these cells into a mouse to produce ascites.
- Reactivity
- Human, Mouse, Rat
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- TLL6C5
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Thalamocortical neurons display suppressed burst-firing due to an enhanced Ih current in a genetic model of absence epilepsy.
HCN channels are expressed differentially in retinal bipolar cells and concentrated at synaptic terminals.
Hyperpolarization-activated channels HCN1 and HCN4 mediate responses to sour stimuli.
Cain SM, Tyson JR, Jones KL, Snutch TP
Pflugers Archiv : European journal of physiology 2015 Jun;467(6):1367-82
Pflugers Archiv : European journal of physiology 2015 Jun;467(6):1367-82
HCN channels are expressed differentially in retinal bipolar cells and concentrated at synaptic terminals.
Müller F, Scholten A, Ivanova E, Haverkamp S, Kremmer E, Kaupp UB
The European journal of neuroscience 2003 May;17(10):2084-96
The European journal of neuroscience 2003 May;17(10):2084-96
Hyperpolarization-activated channels HCN1 and HCN4 mediate responses to sour stimuli.
Stevens DR, Seifert R, Bufe B, Müller F, Kremmer E, Gauss R, Meyerhof W, Kaupp UB, Lindemann B
Nature 2001 Oct 11;413(6856):631-5
Nature 2001 Oct 11;413(6856):631-5
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 HCN protein isoforms are differentially expressed in GAERS VB thalamus. a Representative Western blots for HCN proteins from adult NEC and GAERS VB samples. Primary antibodies to detect each of the HCN channel isoforms were compared to vinculin in order to normalize the expression data. b Mean Western blot densitometry data for HCN1-4 normalized to vinculin for NEC ( n = 12 samples (six animals); black columns ) and GAERS ( n = 12 samples (six animals); grey columns ) VB samples
