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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
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- Product number
- PA5-120727 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SSR2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive test controls include: HepG2, A-549, SKOV3, Mouse liver, Mouse pancreas, Rat liver, Rat pancreas. Immunogen sequence: EEGARLLASK SLLNRYAVEG RDLTLQYNIY NVGSSAALDV ELSDDSFPPE DFGIVSGMLN VKWDRIAPAS NVSHTVVLRP LKAGYFNFTS ATITYLAQED GPVVIGSTSA PGQGGILAQR EFDRRFSP
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.60 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SSR2 in extracts of various cell lines. Samples were incubated with SSR2 Polyclonal antibody (Product # PA5-120727) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of SSR2 in C6 cells. Samples were incubated with SSR2 Polyclonal antibody (Product # PA5-120727) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of SSR2 in NIH/3T3 cells. Samples were incubated with SSR2 Polyclonal antibody (Product # PA5-120727) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SSR2 in paraffin-embedded rat testis. Samples were incubated with SSR2 Polyclonal antibody (Product # PA5-120727) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SSR2 in paraffin-embedded mouse brain. Samples were incubated with SSR2 Polyclonal antibody (Product # PA5-120727) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SSR2 in paraffin-embedded rat testis. Samples were incubated with SSR2 Polyclonal antibody (Product # PA5-120727) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SSR2 in paraffin-embedded mouse brain. Samples were incubated with SSR2 Polyclonal antibody (Product # PA5-120727) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
