PA5-118254

antibody from Invitrogen Antibodies

Targeting: SEMA4A CORD10, FLJ12287, SEMAB, SemB

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118254

Provider

Invitrogen Antibodies

Product name

SEMA4A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of SEMA4A in Lane A: 0.5 mg Jurkat Whole Cell Lysate. Samples were treated with 15 μl of 50 % Protein G agarose, incubated with polyclonal antibody (Product # PA5-118254) with a dilution of 1:500, followed by Dylight 800-labeled antibody to rabbit IgG (H+L) using a dilution of 1:5,000. Assay was performed under reducing conditions. Predicted band size: 84 kDa, Observed band size: 105 kDa .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SEMA4A Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 0.5 µL with SEMA4A Polyclonal Antibody (Product # PA5-118254) and 15 µL of 50 % Protein G agarose. Primary antibody: SEMA4A Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 84 kDa. Observed band size: 105 kDa.