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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- RQ8233 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Semaphorin 4A Antibody / SEMA4A / Sema B
- Antibody type
- Polyclonal
- Description
- This highly specific Semaphorin 4A antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Semaphorin 4A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) human Jurkat, 3) human RT4, 4) rat thymus, 5) rat PC-12, 6) mouse thymus and 7) mouse ANA-1 cell lysate with Semaphorin 4A antibody. Predicted molecular weight ~84 kDa but may be observed at higher molecular weights due to glycosylation.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human JK cells with Semaphorin 4A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Semaphorin 4A antibody.
