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- References [17]
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- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- 17719-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#17719-1-AP, RRID:AB_2223896
- Product name
- P5CS antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated P5CS antibody (Cat. #17719-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Carnosol ameliorated cancer cachexia-associated myotube atrophy by targeting P5CS and its downstream pathways.
Loss of attachment promotes proline accumulation and excretion in cancer cells.
IGF1R-phosphorylated PYCR1 facilitates ELK4 transcriptional activity and sustains tumor growth under hypoxia.
Single-Cell Metabolomics-Based Strategy for Studying the Mechanisms of Drug Action.
A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy.
Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis.
Inhibition of the proline metabolism rate-limiting enzyme P5CS allows proliferation of glutamine-restricted cancer cells.
Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling.
SLC25A51 promotes tumor growth through sustaining mitochondria acetylation homeostasis and proline biogenesis.
Cancer-associated fibroblasts require proline synthesis by PYCR1 for the deposition of pro-tumorigenic extracellular matrix.
PINCH-1 promotes Δ(1)-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma.
Mitochondrial NADP(+) is essential for proline biosynthesis during cell growth.
Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.
Glutamine Metabolism Is Required for Collagen Protein Synthesis in Lung Fibroblasts.
Kindlin-2 links mechano-environment to proline synthesis and tumor growth.
Proline biosynthesis augments tumor cell growth and aerobic glycolysis: involvement of pyridine nucleotides.
Reprogramming of proline and glutamine metabolism contributes to the proliferative and metabolic responses regulated by oncogenic transcription factor c-MYC.
Fang QY, Wang YP, Zhang RQ, Fan M, Feng LX, Guo XD, Cheng CR, Zhang XW, Liu X
Frontiers in pharmacology 2023;14:1291194
Frontiers in pharmacology 2023;14:1291194
Loss of attachment promotes proline accumulation and excretion in cancer cells.
Pilley SE, Hennequart M, Vandekeere A, Blagih J, Legrave NM, Fendt SM, Vousden KH, Labuschagne CF
Science advances 2023 Sep 8;9(36):eadh2023
Science advances 2023 Sep 8;9(36):eadh2023
IGF1R-phosphorylated PYCR1 facilitates ELK4 transcriptional activity and sustains tumor growth under hypoxia.
Zheng K, Sha N, Hou G, Leng Z, Zhao Q, Zhang L, He L, Xu M, Jiang Y, Chen T
Nature communications 2023 Sep 30;14(1):6117
Nature communications 2023 Sep 30;14(1):6117
Single-Cell Metabolomics-Based Strategy for Studying the Mechanisms of Drug Action.
Zhu G, Zhang W, Zhao Y, Chen T, Yuan H, Liu Y, Guo G, Liu Z, Wang X
Analytical chemistry 2023 Mar 14;95(10):4712-4720
Analytical chemistry 2023 Mar 14;95(10):4712-4720
A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy.
Southwell N, Primiano G, Nadkarni V, Attarwala N, Beattie E, Miller D, Alam S, Liparulo I, Shurubor YI, Valentino ML, Carelli V, Servidei S, Gross SS, Manfredi G, Chen Q, D'Aurelio M
EMBO molecular medicine 2023 Jul 10;15(7):e16951
EMBO molecular medicine 2023 Jul 10;15(7):e16951
Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis.
Bai J, Liu T, Tu B, Yuan M, Shu Z, Fan M, Huo S, Guo Y, Wang L, Wang H, Zhao Y
Autophagy 2023 Feb;19(2):632-643
Autophagy 2023 Feb;19(2):632-643
Inhibition of the proline metabolism rate-limiting enzyme P5CS allows proliferation of glutamine-restricted cancer cells.
Linder SJ, Bernasocchi T, Martínez-Pastor B, Sullivan KD, Galbraith MD, Lewis CA, Ferrer CM, Boon R, Silveira GG, Cho HM, Vidoudez C, Shroff S, Oliveira-Costa JP, Ross KN, Massri R, Matoba Y, Kim E, Rueda BR, Stott SL, Gottlieb E, Espinosa JM, Mostoslavsky R
Nature metabolism 2023 Dec;5(12):2131-2147
Nature metabolism 2023 Dec;5(12):2131-2147
Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling.
Key J, Gispert S, Koepf G, Steinhoff-Wagner J, Reichlmeir M, Auburger G
International journal of molecular sciences 2023 Dec 15;24(24)
International journal of molecular sciences 2023 Dec 15;24(24)
SLC25A51 promotes tumor growth through sustaining mitochondria acetylation homeostasis and proline biogenesis.
Li Y, Bie J, Zhao L, Song C, Zhang T, Li M, Yang C, Luo J
Cell death and differentiation 2023 Aug;30(8):1916-1930
Cell death and differentiation 2023 Aug;30(8):1916-1930
Cancer-associated fibroblasts require proline synthesis by PYCR1 for the deposition of pro-tumorigenic extracellular matrix.
Kay EJ, Paterson K, Riera-Domingo C, Sumpton D, Däbritz JHM, Tardito S, Boldrini C, Hernandez-Fernaud JR, Athineos D, Dhayade S, Stepanova E, Gjerga E, Neilson LJ, Lilla S, Hedley A, Koulouras G, McGregor G, Jamieson C, Johnson RM, Park M, Kirschner K, Miller C, Kamphorst JJ, Loayza-Puch F, Saez-Rodriguez J, Mazzone M, Blyth K, Zagnoni M, Zanivan S
Nature metabolism 2022 Jun;4(6):693-710
Nature metabolism 2022 Jun;4(6):693-710
PINCH-1 promotes Δ(1)-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma.
Cui C, Wang J, Guo L, Wu C
Amino acids 2021 Dec;53(12):1875-1890
Amino acids 2021 Dec;53(12):1875-1890
Mitochondrial NADP(+) is essential for proline biosynthesis during cell growth.
Tran DH, Kesavan R, Rion H, Soflaee MH, Solmonson A, Bezwada D, Vu HS, Cai F, Phillips JA 3rd, DeBerardinis RJ, Hoxhaj G
Nature metabolism 2021 Apr;3(4):571-585
Nature metabolism 2021 Apr;3(4):571-585
Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.
Liu M, Wang Y, Yang C, Ruan Y, Bai C, Chu Q, Cui Y, Chen C, Ying G, Li B
The Journal of experimental medicine 2020 Mar 2;217(3)
The Journal of experimental medicine 2020 Mar 2;217(3)
Glutamine Metabolism Is Required for Collagen Protein Synthesis in Lung Fibroblasts.
Hamanaka RB, O'Leary EM, Witt LJ, Tian Y, Gökalp GA, Meliton AY, Dulin NO, Mutlu GM
American journal of respiratory cell and molecular biology 2019 Nov;61(5):597-606
American journal of respiratory cell and molecular biology 2019 Nov;61(5):597-606
Kindlin-2 links mechano-environment to proline synthesis and tumor growth.
Guo L, Cui C, Zhang K, Wang J, Wang Y, Lu Y, Chen K, Yuan J, Xiao G, Tang B, Sun Y, Wu C
Nature communications 2019 Feb 19;10(1):845
Nature communications 2019 Feb 19;10(1):845
Proline biosynthesis augments tumor cell growth and aerobic glycolysis: involvement of pyridine nucleotides.
Liu W, Hancock CN, Fischer JW, Harman M, Phang JM
Scientific reports 2015 Nov 24;5:17206
Scientific reports 2015 Nov 24;5:17206
Reprogramming of proline and glutamine metabolism contributes to the proliferative and metabolic responses regulated by oncogenic transcription factor c-MYC.
Liu W, Le A, Hancock C, Lane AN, Dang CV, Fan TW, Phang JM
Proceedings of the National Academy of Sciences of the United States of America 2012 Jun 5;109(23):8983-8
Proceedings of the National Academy of Sciences of the United States of America 2012 Jun 5;109(23):8983-8
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 17719-1-AP(ALDH18A1 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The P5CS antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human P5CS. This antibody recognizes human,mouse,rat antigen. The P5CS antibody has been validated for the following applications: ELISA, WB, IP analysis.
