17719-1-AP

antibody from Proteintech Group

Targeting: ALDH18A1 GSAS, P5CS, PYCS, SPG9

Provider product page for 17719-1-AP

Western blot

ELISA

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [32]
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Validations
Western blot [1]
Immunoprecipitation [1]

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Product number: 17719-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#17719-1-AP, RRID:AB_2223896
Product name: P5CS antibody
Antibody type: Polyclonal
Description: KD/KO validated P5CS antibody (Cat. #17719-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

ALDH18A1 protein structure - 17719-1-AP shown in red.

Submitted references Topology scaffolds-enhanced paracrine of BMSCs through mechanotransduction-related metabolism reprogramming for burn wounds healing.

Zhang Q, Li J, Jia J, He S, Mei A, Zhang Z, Cao Y, Zhang X, Zhang Y, Li Z, Luo G
Biomaterials 2026 Jan;324:123518

Mitochondrial TRPV1 exacerbates cognitive deficits in sepsis-associated encephalopathy by driving microglial metabolic reprogramming.

Fan Z, Du L, Yin L, Liu H, Li M, Li J, Liu F, Yang L, Li J, Wang Q, Wu Y, Ma H, Zhang X
Free radical biology & medicine 2026 Apr;247:448-468

Identification and Characterization of Genes Associated with Intestinal Ischemia-Reperfusion Injury and Oxidative Stress: A Bioinformatics and Experimental Approach Integrating High-Throughput Sequencing, Machine Learning, and Validation.

Xie Y, Yang M, Huang J, Jiang Z
Journal of inflammation research 2025;18:701-722

Clinical, neuropathological, and biochemical characterization of ALS in a large CHCHD10 R15L family.

Kwan JY, Lantz CI, Korobeynikov VA, Snyder A, Huang X, Haselhuhn T, Dore KN, Madruga A, Danielian LE, Schindler AB, Chia R, Rasheed M, Crook J, Szabo M, Portley M, Sherer CM, King MC, Huang TH, Kosa P, Bielekova B, Ward ME, Grunseich C, Shneider NA, Traynor BJ, Narendra DP
medRxiv : the preprint server for health sciences 2025 Sep 26;

Impaired xCT-mediated cystine uptake drives serine and proline metabolic reprogramming and mitochondrial fission in skeletal muscle cells.

Kanaan MN, Karam CY, Kennedy LS, Pileggi CA, Hamilton L, Cuperlovic-Culf M, Harper ME
Redox biology 2025 Oct;86:103839

Glutamine metabolism reprogramming promotes bladder cancer progression via PYCR1: a multi-omics and functional validation study.

Ding X, Zhang E, Huang Z, Li X, Wang Z, Wu Y, Liu C, Wu S
Journal of translational medicine 2025 Nov 13;23(1):1277

Conversion of glutamate into proline by the leucine analog BCH enhances biphasic insulin secretion in pancreatic β-cells.

Gheibi S, Cataldo LR, Ardalani H, Nocquet L, Spégel P, Straub SG, Sharp GWG, Fex M, Mulder H
The Journal of biological chemistry 2025 May;301(5):108449

Role of GATM/creatine as energy reserves in the poll gland and rutting activities in male Bactrian camel.

Tang Z, Li J, Zhou B, Zhang B, Zhang J, Cheng S, Dong W, Zhang Y, Zhao X, Zhang Q, Gao Y
International journal of biological macromolecules 2025 Jul;318(Pt 2):145042

Metabolic imprinting drives epithelial memory during mucosal fungal infection.

Sekar J, Solis NV, Miao J, Millet N, Tom B, Quintanilla D, Pellon A, Moyes DL, Gogos JA, Rossiter HB, Filler SG, Netea MG, Yee JK, Swidergall M
bioRxiv : the preprint server for biology 2025 Jul 17;

P5CS deacetylation mediated by SIRT2 facilitates tumor growth by enhancing mitochondrial respiration in hepatocellular carcinoma.

Geng X, Li M, Zhang L, Cai Y, Chen X, Mu X, Wang J, Liu B
Oncogene 2025 Aug;44(31):2746-2761

ALDH18A1 has carcinogenic functions and regulates alternative splicing events of DNA repair-related genes in esophageal carcinoma cells.

Yongkang W, Yisireyili M, Abudureyimu K, Enomoto A
Scientific reports 2025 Aug 7;15(1):28845

Tanshinone IIA alleviates pulmonary fibrosis by modulating glutamine metabolic reprogramming based on [U-(13)C(5)]-glutamine metabolic flux analysis.

Shan B, Guo C, Zhou H, Chen J
Journal of advanced research 2025 Apr;70:531-544

Cellular ATP demand creates metabolically distinct subpopulations of mitochondria.

Ryu KW, Fung TS, Baker DC, Saoi M, Park J, Febres-Aldana CA, Aly RG, Cui R, Sharma A, Fu Y, Jones OL, Cai X, Pasolli HA, Cross JR, Rudin CM, Thompson CB
Nature 2024 Nov;635(8039):746-754

Mitochondrial NADK2-dependent NADPH controls Tau oligomer uptake in human neurons.

Pardo E, Kim T, Wallrabe H, Zengeler KE, Sagar VK, Mingledorff G, Sun X, Periasamy A, Lukens JR, Bloom GS, Norambuena A
bioRxiv : the preprint server for biology 2024 Nov 1;

Deciphering glutamine metabolism patterns for malignancy and tumor microenvironment in clear cell renal cell carcinoma.

Wu G, Li T, Chen Y, Ye S, Zhou S, Tian X, Anwaier A, Zhu S, Xu W, Hao X, Ye D, Zhang H
Clinical and experimental medicine 2024 Jul 6;24(1):152

Carnosol ameliorated cancer cachexia-associated myotube atrophy by targeting P5CS and its downstream pathways.

Fang QY, Wang YP, Zhang RQ, Fan M, Feng LX, Guo XD, Cheng CR, Zhang XW, Liu X
Frontiers in pharmacology 2023;14:1291194

Loss of attachment promotes proline accumulation and excretion in cancer cells.

Pilley SE, Hennequart M, Vandekeere A, Blagih J, Legrave NM, Fendt SM, Vousden KH, Labuschagne CF
Science advances 2023 Sep 8;9(36):eadh2023

IGF1R-phosphorylated PYCR1 facilitates ELK4 transcriptional activity and sustains tumor growth under hypoxia.

Zheng K, Sha N, Hou G, Leng Z, Zhao Q, Zhang L, He L, Xu M, Jiang Y, Chen T
Nature communications 2023 Sep 30;14(1):6117

Single-Cell Metabolomics-Based Strategy for Studying the Mechanisms of Drug Action.

Zhu G, Zhang W, Zhao Y, Chen T, Yuan H, Liu Y, Guo G, Liu Z, Wang X
Analytical chemistry 2023 Mar 14;95(10):4712-4720

A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy.

Southwell N, Primiano G, Nadkarni V, Attarwala N, Beattie E, Miller D, Alam S, Liparulo I, Shurubor YI, Valentino ML, Carelli V, Servidei S, Gross SS, Manfredi G, Chen Q, D'Aurelio M
EMBO molecular medicine 2023 Jul 10;15(7):e16951

Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis.

Bai J, Liu T, Tu B, Yuan M, Shu Z, Fan M, Huo S, Guo Y, Wang L, Wang H, Zhao Y
Autophagy 2023 Feb;19(2):632-643

Inhibition of the proline metabolism rate-limiting enzyme P5CS allows proliferation of glutamine-restricted cancer cells.

Linder SJ, Bernasocchi T, Martínez-Pastor B, Sullivan KD, Galbraith MD, Lewis CA, Ferrer CM, Boon R, Silveira GG, Cho HM, Vidoudez C, Shroff S, Oliveira-Costa JP, Ross KN, Massri R, Matoba Y, Kim E, Rueda BR, Stott SL, Gottlieb E, Espinosa JM, Mostoslavsky R
Nature metabolism 2023 Dec;5(12):2131-2147

Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling.

Key J, Gispert S, Koepf G, Steinhoff-Wagner J, Reichlmeir M, Auburger G
International journal of molecular sciences 2023 Dec 15;24(24)

SLC25A51 promotes tumor growth through sustaining mitochondria acetylation homeostasis and proline biogenesis.

Li Y, Bie J, Zhao L, Song C, Zhang T, Li M, Yang C, Luo J
Cell death and differentiation 2023 Aug;30(8):1916-1930

Cancer-associated fibroblasts require proline synthesis by PYCR1 for the deposition of pro-tumorigenic extracellular matrix.

Kay EJ, Paterson K, Riera-Domingo C, Sumpton D, Däbritz JHM, Tardito S, Boldrini C, Hernandez-Fernaud JR, Athineos D, Dhayade S, Stepanova E, Gjerga E, Neilson LJ, Lilla S, Hedley A, Koulouras G, McGregor G, Jamieson C, Johnson RM, Park M, Kirschner K, Miller C, Kamphorst JJ, Loayza-Puch F, Saez-Rodriguez J, Mazzone M, Blyth K, Zagnoni M, Zanivan S
Nature metabolism 2022 Jun;4(6):693-710

PINCH-1 promotes Δ(1)-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma.

Cui C, Wang J, Guo L, Wu C
Amino acids 2021 Dec;53(12):1875-1890

Mitochondrial NADP(+) is essential for proline biosynthesis during cell growth.

Tran DH, Kesavan R, Rion H, Soflaee MH, Solmonson A, Bezwada D, Vu HS, Cai F, Phillips JA 3rd, DeBerardinis RJ, Hoxhaj G
Nature metabolism 2021 Apr;3(4):571-585

Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.

Liu M, Wang Y, Yang C, Ruan Y, Bai C, Chu Q, Cui Y, Chen C, Ying G, Li B
The Journal of experimental medicine 2020 Mar 2;217(3)

Glutamine Metabolism Is Required for Collagen Protein Synthesis in Lung Fibroblasts.

Hamanaka RB, O'Leary EM, Witt LJ, Tian Y, Gökalp GA, Meliton AY, Dulin NO, Mutlu GM
American journal of respiratory cell and molecular biology 2019 Nov;61(5):597-606

Kindlin-2 links mechano-environment to proline synthesis and tumor growth.

Guo L, Cui C, Zhang K, Wang J, Wang Y, Lu Y, Chen K, Yuan J, Xiao G, Tang B, Sun Y, Wu C
Nature communications 2019 Feb 19;10(1):845

Proline biosynthesis augments tumor cell growth and aerobic glycolysis: involvement of pyridine nucleotides.

Liu W, Hancock CN, Fischer JW, Harman M, Phang JM
Scientific reports 2015 Nov 24;5:17206

Reprogramming of proline and glutamine metabolism contributes to the proliferative and metabolic responses regulated by oncogenic transcription factor c-MYC.

Liu W, Le A, Hancock C, Lane AN, Dang CV, Fan TW, Phang JM
Proceedings of the National Academy of Sciences of the United States of America 2012 Jun 5;109(23):8983-8

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: MCF7 cells were subjected to SDS PAGE followed by western blot with 17719-1-AP(ALDH18A1 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The P5CS antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human P5CS. This antibody recognizes human,mouse,rat antigen. The P5CS antibody has been validated for the following applications: ELISA, WB, IP analysis.
Appendix: 17719-1-AP.pdf

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