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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-52546 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALDH18A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SIVEQVSVLQ NQGREMMLVT SGAVAFGKQR LRHEILLSQS VRQALHSGQN QLKEMAIPVL EARACAAAGQ SGLMALYEAM FTQYSICAAQ ILVTNLDFHD EQKRRNLNGT LHELLRMNIV PIVNTNDAVV PPAEPNSDLQ GVNVISVKD Highest antigen sequence identity to the following orthologs: Mouse - 99%, Rat - 99%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Utility and Mechanism of SHetA2 and Paclitaxel for Treatment of Endometrial Cancer.
Chandra V, Rai R, Benbrook DM
Cancers 2021 May 12;13(10)
Cancers 2021 May 12;13(10)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ALDH18A1 in mouse cell line NIH-3T3 and rat cell line NBT-II using a ALDH18A1 Polyclonal Antibody (Product # PA5-52546).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ALDH18A1 using ALDH18A1 Polyclonal Antibody (Product # PA5-52546) (A) shows similar pattern to an independent ALDH18A1 Polyclonal Antibody (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ALDH18A1 in human cell line U-2 OS shows positivity in mitochondria. Samples were probed using an ALDH18A1 Polyclonal Antibody (Product # PA5-52546).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ALDH18A1 in human colon tissue shows strong cytoplasmic positivity in glandular cells. Samples were probed using an ALDH18A1 Polyclonal Antibody (Product # PA5-52546).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Molecular mechanism of SHetA2: ( A ) Ishikawa cells were treated with SHetA2 (10 uM) for 4 h and co-immunoprecipitation was performed with mortalin antibody (Grp75)-tagged beads. Western blots (left panel) and densitometry analyses (right panel) was performed for IP3R, ALDH18A1, CTPS, MDH1, and ECHS1 to confirm the reduction of client protein co-immunoprecipitation. ( B ) Western blot analysis for the protein expression of IP3R, Cl. PARP, total caspase-3, P62, AIF, DNA damage marker (gammaH2AX) and LC3-II proteins in endometrial cancer cells treated with, or without, SHetA2 (10 uM) for 24 h. ( C ) Cell apoptosis was detected by Annexin-V/PI combined labeling with flow cytometry in endometrial cancer cells 24 h after treatment with SHetA2 (10 uM) or vehicle. ( D ) Caspase-3 activity assay for endometrial cancer cells treated with indicated dose of SHetA2 for 24 h. ** p
