Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-83325 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-83325, RRID:AB_11003901
- Product name
- Rabbit Polyclonal ALDH18A1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human ALDH18A1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Nuclear cytoplasmic trafficking of proteins is a major response of human fibroblasts to oxidative stress.
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
Baqader NO, Radulovic M, Crawford M, Stoeber K, Godovac-Zimmermann J
Journal of proteome research 2014 Oct 3;13(10):4398-423
Journal of proteome research 2014 Oct 3;13(10):4398-423
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of proteomics 2012 Apr 3;75(7):2236-51
Journal of proteomics 2012 Apr 3;75(7):2236-51
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ALDH18A1 Antibody [NBP1-83325] - Analysis using Anti-ALDH18A1 antibody NBP1-83325 (A) shows similar pattern to independent antibody NBP1-83324 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ALDH18A1 Antibody [NBP1-83325] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ALDH18A1 Antibody [NBP1-83325] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ALDH18A1 Antibody [NBP1-83325] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.