H00001760-M01

antibody from Invitrogen Antibodies

Targeting: DMPK DM, DM1, DM1PK, DMK, MDPK, MT-PK

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001760-M01

Provider

Invitrogen Antibodies

Product name

DMPK Monoclonal Antibody (2F7)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Sequence of this protein is as follows: DEGVPEEARD FIQRLLCPPE TRLGRGGAGD FRTHPFFFGL DWDGLRDSVP PFTPDFEGAT DTCNFDLVED GLTAMVSGGG ETLSDIREGA PLGVHLPFVG YSYSCMALRD SEVPGPTP

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

2F7

Vial size

100 µg

Concentration

See Label

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of DMPK expression in transfected 293T cell line by DMPK monoclonal antibody (M01), clone 2F7.Lane 1: DMPK transfected lysate(69.4 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DMPK over-expressed 293 cell line, cotransfected with DMPK Validated Chimera RNAi (Cat # H00001760-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DMPK monoclonal antibody (M01) clone 2F7 (Cat # H00001760-M01). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of DMPK expression in transfected 293T cell line by DMPK monoclonal antibody (M01), clone 2F7.Lane 1: DMPK transfected lysate(69.4 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged DMPK is approximately 0.03 ng/mL as a capture antibody.