10225-1-AP

antibody from Proteintech Group

Targeting: CRABP2 CRABP-II

Provider product page for 10225-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [55]
Comments [0]
Validations
Western blot [1]
Immunoprecipitation [1]
Immunohistochemistry [1]

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Product number: 10225-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10225-1-AP, RRID:AB_2085455
Product name: CRABP2 antibody
Antibody type: Polyclonal
Description: KD/KO validated CRABP2 antibody (Cat. #10225-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

CRABP2 protein structure - 10225-1-AP shown in red.

Submitted references FOXC1 Regulates Cytokine Signaling, Inflammatory Pathways, and Retinoid Metabolism to Maintain Limbal Epithelial Cell Homeostasis In Vitro.

Kundu S, Amini M, Stachon T, Fries FN, Seitz B, Li Z, Li S, Liu S, Hsu SL, Suiwal S, Szentmáry N
International journal of molecular sciences 2026 Feb 15;27(4)

CRABP2 (Cellular Retinoic Acid Binding Protein 2D): A novel biomarker for the diagnosis and prognosis involved in immune infiltration of lung adenocarcinoma.

Cai D, Tian F, Zhang D, Tu J, Wang Y
Journal of Cancer 2025;16(5):1631-1646

Canagliflozin alleviates progestin resistance by suppressing RARβ/CRABP2 signaling in THRB knockout endometrial cancer cells.

Yang Y, Zhou J, Lv Q, Ni Q, Hu B, Wang Y, Qu S, Li G, Yang W, Zhong R, Chen X, Zhu Y
Frontiers in pharmacology 2025;16:1573032

Identification of a Treg-related gene signature for predicting prognosis and immunosuppression in skin cutaneous melanoma.

Lian C, Jin R, Zhang X
Clinical and experimental medicine 2025 Nov 25;26(1):35

UTX (KDM6A) promotes differentiation noncatalytically in somatic self-renewing epithelia.

Pacella GN, Kuprasertkul N, Bao L, Huang S, D'souza C, Prouty SM, Anderson A, Maldonado López AM, Sinkfield M, Olingou C, Seykora JT, Capell BC
Proceedings of the National Academy of Sciences of the United States of America 2025 May 20;122(20):e2422971122

Dual Role of CRABP2 in Colorectal Cancer: Oncogenesis via Nuclear RB1 and Cytoplasmic AFG3L2/SLC25A39 Axis, While Limiting Liver Metastasis through Cytoplasmic AFG3L2/PINK1/Parkin-Mediated Mitophagy.

Tian C, Yang S, Zhang C, Zhu R, Chen C, Wang X, Zhang D, Sun Q, Xu H, Nie H, Zhang Y, Ji D, Tang J, Jin K, Sun Y
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Jun;12(23):e2500552

Identification of differentiation markers and immune microenvironment in head and neck squamous cell carcinoma using machine learning combined with single-cell analysis.

Zhang Z, Li Y, Wang M, Jing Y, Hu H, Shi M, Ding Y, Chen X
Translational cancer research 2025 Dec 31;14(12):8579-8599

SPP1(+) Venous Endothelial Cells and CRABP2(+) Tumor Cells Contribute to Favorable Body and Tail Pancreatic Ductal Adenocarcinoma Tumor Microenvironment.

Liu Y, Fu Y, Liu T, Wang J, Zhang Z, Shi Y, Cao Z, Yang G, Chen H, Luo W, Tao J, Wang Y, Weng G, Zhang M, Ye L, Xiao J, Qiu J, Zhang T, Huang H
Molecular carcinogenesis 2025 Aug;64(8):1377-1391

The mediating mechanism of Oct4 in the process of cleft palate induced by 2, 3, 7, 8-tetrachlorodibenz-p-dioxin (TCDD) and all-trans retinoic acid.

Yu Z, Zhang Y, Wu S, Sun Z, Gao Z, Chen Y, Wang X, Xu D, Liu X
Ecotoxicology and environmental safety 2025 Apr 15;295:118102

Heterogeneous fibroblasts contribute to fibrotic scar formation after spinal cord injury in mice and monkeys.

Xue X, Wu X, Fan Y, Han S, Zhang H, Sun Y, Yin Y, Yin M, Chen B, Sun Z, Zhao S, Zhang Q, Liu W, Zhang J, Li J, Shi Y, Xiao Z, Dai J, Zhao Y
Nature communications 2024 Jul 27;15(1):6321

The chromatin regulator Ankrd11 controls cardiac neural crest cell-mediated outflow tract remodeling and heart function.

Kibalnyk Y, Afanasiev E, Noble RMN, Watson AES, Poverennaya I, Dittmann NL, Alexiou M, Goodkey K, Greenwell AA, Ussher JR, Adameyko I, Massey J, Graf D, Bourque SL, Stratton JA, Voronova A
Nature communications 2024 Jul 1;15(1):4632

GRPR Drives Metastasis via CRABP2 and FNDC4 Pathways in Lung Adenocarcinoma.

Kim DG, Choi EY, Ahn HM, Kim YJ
Cells 2024 Dec 23;13(24)

Overcoming retinoic acid resistance in HER2-enriched breast cancers: role of MYC.

Choi WS, Liu RZ, Mak C, Maadi H, Godbout R
The FEBS journal 2024 Aug;291(15):3521-3538

Disrupted protein interaction dynamics in a genetic neurodevelopmental disorder revealed by structural bioinformatics and genetic code expansion.

Marino V, Phromkrasae W, Bertacchi M, Cassini P, Chakrabandhu K, Dell'Orco D, Studer M
Protein science : a publication of the Protein Society 2024 Apr;33(4):e4953

METTL3-mediated RanGAP1 promotes colorectal cancer progression through the MAPK pathway by recruiting YTHDF1.

Yang R, Yang C, Su D, Song Y, Min J, Qian Z, Shen X, Li J, Su H
Cancer gene therapy 2024 Apr;31(4):562-573

CRABP2 reduces the sensitivity of Olaparib in ovarian cancer by downregulating Caspase-8 and decreasing the production of reactive oxygen species.

Zeng S, Xu Z, Liu Y, Zhou S, Yan Y
Chemico-biological interactions 2024 Apr 25;393:110958

CRABP2 regulates infiltration of cancer-associated fibroblasts and immune response in melanoma.

Zeng S, Chen XI, Yi Q, Thakur A, Yang H, Yan Y, Liu S
Oncology research 2023;32(2):261-272

Depletion of LONP2 unmasks differential requirements for peroxisomal function between cell types and in cholesterol metabolism.

Yamashita A, Ignatenko O, Nguyen M, Lambert R, Watt K, Daneault C, Robillard-Frayne I, Topisirovic I, Rosiers CD, McBride HM
Biology direct 2023 Sep 22;18(1):60

Structural characterization of SLYM - a 4th meningeal membrane.

Plá V, Bitsika S, Giannetto M, Ladron-de-Guevara A, Gahn-Martinez D, Mori Y, Nedergaard M, Møllgård K
Research square 2023 Oct 31;

The heterogeneity of tumour immune microenvironment revealing the CRABP2/CD69 signature discriminates distinct clinical outcomes in breast cancer.

Mei J, Cai Y, Chen L, Wu Y, Liu J, Qian Z, Jiang Y, Zhang P, Xia T, Pan X, Zhang Y
British journal of cancer 2023 Nov;129(10):1645-1657

The prognostic gene CRABP2 affects drug sensitivity by regulating docetaxel-induced apoptosis in breast invasive carcinoma: A pan-cancer analysis.

Zeng S, Xu Z, Liang Q, Thakur A, Liu Y, Zhou S, Yan Y
Chemico-biological interactions 2023 Mar 1;373:110372

Molecular anatomy of adult mouse leptomeninges.

Pietilä R, Del Gaudio F, He L, Vázquez-Liébanas E, Vanlandewijck M, Muhl L, Mocci G, Bjørnholm KD, Lindblad C, Fletcher-Sandersjöö A, Svensson M, Thelin EP, Liu J, van Voorden AJ, Torres M, Antila S, Xin L, Karlström H, Storm-Mathisen J, Bergersen LH, Moggio A, Hansson EM, Ulvmar MH, Nilsson P, Mäkinen T, Andaloussi Mäe M, Alitalo K, Proulx ST, Engelhardt B, McDonald DM, Lendahl U, Andrae J, Betsholtz C
Neuron 2023 Dec 6;111(23):3745-3764.e7

Structural characterization of SLYM-a 4th meningeal membrane.

Plá V, Bitsika S, Giannetto MJ, Ladron-de-Guevara A, Gahn-Martinez D, Mori Y, Nedergaard M, Møllgård K
Fluids and barriers of the CNS 2023 Dec 14;20(1):93

Differential Regulation of Retinoic Acid Metabolism in Fanconi Anemia.

Blaize JL, Noori BM, Hunter KP, Henrikson KA, Atoyan JA, Ardito AA, Donovan FX, Chandrasekharappa SC, Schindler D, Howlett NG
bioRxiv : the preprint server for biology 2023 Apr 27;

Effects of Fatty Acids on Proliferation of Cultured Wild-type and FABP5-KO Thymic Epithelial Cells.

Adachi Y
Journal of UOEH 2022;44(3):239-248

Dezocine inhibits cell proliferation, migration, and invasion by targeting CRABP2 in ovarian cancer.

Zhang C, Pan R, Ma S, Xu S, Wang B
Open medicine (Warsaw, Poland) 2022;17(1):2052-2061

Upregulation of CRABP2 by TET1-mediated DNA hydroxymethylation attenuates mitochondrial apoptosis and promotes oxaliplatin resistance in gastric cancer.

Tang X, Liang Y, Sun G, He Q, Hou Z, Jiang X, Gao P, Qu H
Cell death & disease 2022 Oct 4;13(10):848

Targeting CRABP-II overcomes pancreatic cancer drug resistance by reversing lipid raft cholesterol accumulation and AKT survival signaling.

Yu S, Wang L, Che D, Zhang M, Li M, Naito M, Xin W, Zhou L
Journal of experimental & clinical cancer research : CR 2022 Mar 8;41(1):88

All-trans retinoic acid increases the pathogenicity of the H9N2 influenza virus in mice.

Niu X, Wang H, Zhao L, Lian P, Bai Y, Li J, Qiao J
Virology journal 2022 Jun 28;19(1):113

The growth and expansion of meningeal lymphatic networks are affected in craniosynostosis.

Ang PS, Matrongolo MJ, Tischfield MA
Development (Cambridge, England) 2022 Jan 1;149(1)

The developing mouse coronal suture at single-cell resolution.

Farmer DT, Mlcochova H, Zhou Y, Koelling N, Wang G, Ashley N, Bugacov H, Chen HJ, Parvez R, Tseng KC, Merrill AE, Maxson RE Jr, Wilkie AOM, Crump JG, Twigg SRF
Nature communications 2021 Aug 10;12(1):4797

TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains.

Kaiho-Soma A, Akizuki Y, Igarashi K, Endo A, Shoda T, Kawase Y, Demizu Y, Naito M, Saeki Y, Tanaka K, Ohtake F
Molecular cell 2021 Apr 1;81(7):1411-1424.e7

Single-Cell Transcriptomic Analyses of the Developing Meninges Reveal Meningeal Fibroblast Diversity and Function.

DeSisto J, O'Rourke R, Jones HE, Pawlikowski B, Malek AD, Bonney S, Guimiot F, Jones KL, Siegenthaler JA
Developmental cell 2020 Jul 6;54(1):43-59.e4

Resveratrol Reverses Retinoic Acid Resistance of Anaplastic Thyroid Cancer Cells via Demethylating CRABP2 Gene.

Liu X, Li H, Wu ML, Wu J, Sun Y, Zhang KL, Liu J
Frontiers in endocrinology 2019;10:734

Crabp2 Promotes Metastasis of Lung Cancer Cells via HuR and Integrin β1/FAK/ERK Signaling.

Wu JI, Lin YP, Tseng CW, Chen HJ, Wang LH
Scientific reports 2019 Jan 29;9(1):845

Development of Small Molecule Chimeras That Recruit AhR E3 Ligase to Target Proteins.

Ohoka N, Tsuji G, Shoda T, Fujisato T, Kurihara M, Demizu Y, Naito M
ACS chemical biology 2019 Dec 20;14(12):2822-2832

CRABP2 regulates invasion and metastasis of breast cancer through hippo pathway dependent on ER status.

Feng X, Zhang M, Wang B, Zhou C, Mu Y, Li J, Liu X, Wang Y, Song Z, Liu P
Journal of experimental & clinical cancer research : CR 2019 Aug 16;38(1):361

Resveratrol Suppresses the Growth and Enhances Retinoic Acid Sensitivity of Anaplastic Thyroid Cancer Cells.

Li YT, Tian XT, Wu ML, Zheng X, Kong QY, Cheng XX, Zhu GW, Liu J, Li H
International journal of molecular sciences 2018 Mar 29;19(4)

Quantitative proteomics analysis of the role of tetraspanin-8 in the drug resistance of gastric cancer.

Li L, Yang D, Cui D, Li Y, Nie Z, Wang J, Liang L
International journal of oncology 2018 Feb;52(2):473-484

Differential CRABP-II and FABP5 expression patterns and implications for medulloblastoma retinoic acid sensitivity.

Zhang S, Liu H, Li H, Wu M, Yu Y, Li F, Cheng X
RSC advances 2018 Apr 11;8(25):14048-14055

Retinoic acid regulates cell-shape and -death of E-FABP (FABP5)-immunoreactive septoclasts in the growth plate cartilage of mice.

Bando Y, Yamamoto M, Sakiyama K, Sakashita H, Taira F, Miyake G, Iseki S, Owada Y, Amano O
Histochemistry and cell biology 2017 Sep;148(3):229-238

Divergent Hox Coding and Evasion of Retinoid Signaling Specifies Motor Neurons Innervating Digit Muscles.

Mendelsohn AI, Dasen JS, Jessell TM
Neuron 2017 Feb 22;93(4):792-805.e4

Cellular Retinoic Acid Binding Protein 2 Is Strikingly Downregulated in Human Esophageal Squamous Cell Carcinoma and Functions as a Tumor Suppressor.

Yang Q, Wang R, Xiao W, Sun F, Yuan H, Pan Q
PloS one 2016;11(2):e0148381

Association between cytoplasmic CRABP2, altered retinoic acid signaling, and poor prognosis in glioblastoma.

Liu RZ, Li S, Garcia E, Glubrecht DD, Poon HY, Easaw JC, Godbout R
Glia 2016 Jun;64(6):963-76

Col1a1+ perivascular cells in the brain are a source of retinoic acid following stroke.

Kelly KK, MacPherson AM, Grewal H, Strnad F, Jones JW, Yu J, Pierzchalski K, Kane MA, Herson PS, Siegenthaler JA
BMC neuroscience 2016 Jul 15;17(1):49

Hes1 and Hes5 are required for differentiation of pituicytes and formation of the neurohypophysis in pituitary development.

Goto M, Hojo M, Ando M, Kita A, Kitagawa M, Ohtsuka T, Kageyama R, Miyamoto S
Brain research 2015 Nov 2;1625:206-17

CRABP-II- and FABP5-independent responsiveness of human glioblastoma cells to all-trans retinoic acid.

Xia SL, Wu ML, Li H, Wang JH, Chen NN, Chen XY, Kong QY, Sun Z, Liu J
Oncotarget 2015 Mar 20;6(8):5889-902

BLIMP1 is required for postnatal epidermal homeostasis but does not define a sebaceous gland progenitor under steady-state conditions.

Kretzschmar K, Cottle DL, Donati G, Chiang MF, Quist SR, Gollnick HP, Natsuga K, Lin KI, Watt FM
Stem cell reports 2014 Oct 14;3(4):620-33

Cellular retinoic acid binding protein I mediates rapid non-canonical activation of ERK1/2 by all-trans retinoic acid.

Persaud SD, Lin YW, Wu CY, Kagechika H, Wei LN
Cellular signalling 2013 Jan;25(1):19-25

CRABP-II- and FABP5-independent all-trans retinoic acid resistance in COLO 16 human cutaneous squamous cancer cells.

Chen NN, Li Y, Wu ML, Liu ZL, Fu YS, Kong QY, Chen XY, Li H, Liu J
Experimental dermatology 2012 Jan;21(1):13-8

CRABP-II methylation: a critical determinant of retinoic acid resistance of medulloblastoma cells.

Fu YS, Wang Q, Ma JX, Yang XH, Wu ML, Zhang KL, Kong QY, Chen XY, Sun Y, Chen NN, Shu XH, Li H, Liu J
Molecular oncology 2012 Feb;6(1):48-61

A single progenitor population switches behavior to maintain and repair esophageal epithelium.

Doupé DP, Alcolea MP, Roshan A, Zhang G, Klein AM, Simons BD, Jones PH
Science (New York, N.Y.) 2012 Aug 31;337(6098):1091-3

A role of the LIM-homeobox gene Lhx2 in the regulation of pituitary development.

Zhao Y, Mailloux CM, Hermesz E, Palkóvits M, Westphal H
Developmental biology 2010 Jan 15;337(2):313-23

Quantitative proteome analysis of HCC cell lines with different metastatic potentials by SILAC.

Chen N, Sun W, Deng X, Hao Y, Chen X, Xing B, Jia W, Ma J, Wei H, Zhu Y, Qian X, Jiang Y, He F
Proteomics 2008 Dec;8(23-24):5108-18

Dynamic regulation of retinoic acid-binding proteins in developing, adult and neoplastic skin reveals roles for beta-catenin and Notch signalling.

Collins CA, Watt FM
Developmental biology 2008 Dec 1;324(1):55-67

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 10225-1-AP(CRABP2 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-CRABP2 (IP:10225-1-AP, 3ug; Detection:10225-1-AP 1:300) with HeLa cells lysate 4650ug.
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The CRABP2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human CRABP2. This antibody recognizes human,mouse,rat antigen. The CRABP2 antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.
Appendix: 10225-1-AP.pdf

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