AF5740

antibody from Novus Biologicals

Targeting: CRABP2 CRABP-II

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF5740

Provider

Novus Biologicals

Product name

Goat Polyclonal CRABP2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects endogenous human CRABP2 in Western blots.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Activation of the CRABPII/RAR pathway by curcumin induces retinoic acid mediated apoptosis in retinoic acid resistant breast cancer cells.

Thulasiraman P, Garriga G, Danthuluri V, McAndrews DJ, Mohiuddin IQ
Oncology reports 2017 Apr;37(4):2007-2015

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human CRABP2 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, MDA-MB-468 human breast cancer cell line, and T47D human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human CRABP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5740) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CRABP2 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.