MA3-017

antibody from Invitrogen Antibodies

Targeting: CRABP2 CRABP-II

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA3-017

Provider

Invitrogen Antibodies

Product name

CRABP2 Monoclonal Antibody (5CRA-3B3)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA3-017 has been used successfully in Western blotting and Immunofluorescence. This antibody was referenced in publications to perform in IHC.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

5CRA-3B3

Vial size

50 µL

Concentration

Conc. Not Determined

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CRABP2 was performed by loading 50 µg of the indicated whole cell lysates and 5 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a Novex 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BOX ). Proteins were transferred to a PVDF membrane using the G2 Blotter (Product # 62288), and blocked with Starting Block T20 (Product # 37543) for 1 hour at room temperature. CRABP2 was detected at ~14 kD using a CRABP2 monoclonal antibody (Product # MA3-017) at a dilution of 1:2000 in blocking buffer for 1 hour at room temperature on a rocking platform, followed by a Goat anti-Mouse IgG Secondary Antibody, HRP conjugate (Product # 31430) at a dilution of 1:20,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal Pico substrate (Product # 34078) and the myECL Imager (Product # 62236).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of CRABP2 (green) in U2OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 10 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a CRABP2 monoclonal antibody (Product # MA3-017) at a dilution of 1:100 in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG Secondary Antibody, DyLight 488 conjugate (Product # 35502) at a dilution of 1:500 for 1 hour at room temperature (green). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.