Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- MA5-29188 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CRABP2 Recombinant Rabbit Monoclonal Antibody (4)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
- Antibody clone number
- 4
- Concentration
- 5 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CRABP2 in Lane A: 293T Whole Cell Lysate (30 µg), Lane B: MCF7 Whole Cell Lysate (30 µg) . Samples were probed using a CRABP2 Monoclonal Antibody (Product # MA5-29188) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:16 kDa. Observed band size:14 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CRABP2 Recombinant Rabbit Monoclonal Antibody (4) (Product # MA5-29188) at 1:500 dilution. Lane A: 293T Whole Cell Lysate, Lane B: MCF7 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 14 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of Human CRABP2 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with CRABP2 Recombinant Rabbit Monoclonal Antibody (4) (Product # MA5-29188, 1:300) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Human CRABP2 expression on MCF-7 cells. The cells were treated according to manufacturer’s manual, stained with CRABP2 Recombinant Rabbit Monoclonal Antibody (4) (Product # MA5-29188), then a FITC-conjugated Secondary antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CRABP2 Immunoprecipitation using: Lane A: 0.5 mg 293T Whole Cell Lysate, Lane B: 0.5 mg Raji Whole Cell Lysate 0.5 µL with CRABP2 Recombinant Rabbit Monoclonal Antibody (4) (Product # MA5-29188) and 60 μg of Immunomagnetic beads Protein G. Primary antibody: CRABP2 Recombinant Rabbit Monoclonal Antibody (4), at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 16 kDa.