Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [9]
- Flow cytometry [1]
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Validation data
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- Product number
- TA504364 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504364, RRID:AB_11129410
- Product name
- SH2D2A mouse monoclonal antibody, clone OTI4F3 (formerly 4F3)
- Antibody type
- Monoclonal
- Description
- SH2D2A mouse monoclonal antibody, clone OTI4F3 (formerly 4F3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SH2D2A
- Isotype
- IgG
- Antibody clone number
- OTI4F3
- Vial size
- 100 µl
- Concentration
- 0.71 mg/ml
Submitted references Expression of the T cell-specific adapter protein in human tissues.
Pandya AD, Leergaard TB, Dissen E, Haraldsen G, Spurkland A
Scandinavian journal of immunology 2014 Sep;80(3):169-79
Scandinavian journal of immunology 2014 Sep;80(3):169-79
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH2D2A (RC204162, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH2D2A.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-SH2D2A mouse monoclonal antibody (TA504364) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SH2D2A(RC204162).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SH2D2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504364)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SH2D2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504364)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SH2D2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504364)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SH2D2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504364)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SH2D2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504364)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SH2D2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504364)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SH2D2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504364)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SH2D2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504364)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Comparison of IHC on tonsil with clone 4F3(TA504364) and clone 3C7(TA504372) SH2D2A mAb and HRP-conjugated secondary antibodies.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC204162 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SH2D2A antibody(TA504364), and then analyzed by flow cytometry.
- Validation comment
- FC