AF6020

antibody from R&D Systems

Targeting: SH2D2A F2771, TSAd

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF6020

Provider

R&D Systems

Product name

Human SH2D2A Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human SH2D2A in direct ELISAs and Western blots.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

Q9NP31


Isotype

IgG

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

VEGFR2 induces c-Src signaling and vascular permeability in vivo via the adaptor protein TSAd.

Sun Z, Li X, Massena S, Kutschera S, Padhan N, Gualandi L, Sundvold-Gjerstad V, Gustafsson K, Choy WW, Zang G, Quach M, Jansson L, Phillipson M, Abid MR, Spurkland A, Claesson-Welsh L
The Journal of experimental medicine 2012 Jul 2;209(7):1363-77

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Axl is essential for VEGF-A-dependent activation of PI3K/Akt.

Ruan GX, Kazlauskas A
The EMBO journal 2012 Apr 4;31(7):1692-703

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human SH2D2A by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line (untreated), human primary differentiated Th2 cells and human primary differentiated Th17 cells treated (+) with 1 µg/mL Hamster Anti-Mouse CD3 epsilon Monoclonal Antibody (Catalog # MAB484) and 5 µg/mL Rat Anti-Mouse CD28 Monoclonal Antibody (Catalog # MAB4831) for 120 minutes. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human SH2D2A Polyclonal Antibody (Catalog # AF6020) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SH2D2A at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human SH2D2A by Simple Western^TM. Simple Western lane view shows lysate of human primary differentiated Th2 cells loaded at 0.2 mg/mL. A specific band was detected for SH2D2A at approximately 58 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human SH2D2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6020) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.