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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 15629-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GCK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: EDIDKGILL NWTKGFKASG AEGNNVVGLL RDAIKRRGDF EMDVVAMVND TVATMISCYY EDHQCEVGMI VGTGCNACYM EEMQNVELVE GDEGRMCVNT EWGAFGDSGE LDEFLLEYDR LVDESSANPG QQLYEKLIGG KYMGELVRLV LLRLVDENLL FHGEASEQLR TRGAFETRFV SQVESDTGDR KQIYNILSTL GLRPSTTDCD IVRRACESVS TRAAHMCSAG LAGVINRMRE SRSEDVMRIT VGVDGSVYKL HPSFKERFHA SVRRLTPSCE ITFIESEEGS GRGAALVSAV ACKKACMLGQ (157-465 aa encoded by BC001890)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 15629-1-AP (GCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 15629-1-AP (GCK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using (Product # 15629-1-AP) (GCK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-GCK (IP:15629-1-AP, 4ug; Detection:15629-1-AP 1:500) with mouse liver tissue lysate 7000ug.
