Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 14-0018-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD1b Monoclonal Antibody (eBioSN13 (SN13 K5-1B8)), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioSN13 monoclonal antibody reacts with human CD1b. The CD1 molecules are similar to MHC class I, but rather than presenting peptide antigens to T-cell receptors (TCR), they present a diversity of lipid and glycolipid antigens which are derived from self as well as from mycobacteria which include sphingolipids, mycolic acid derivatives and phosphatidylinositols such as liparabinomannan (LAM) and glucose monomycolate (GMM). CD1b is 45 kDa, and like the other CD1 antigens, is expressed as a heterodimer composed of the CD1b noncovalently paired with beta2-microglobulin. Applications Reported: This eBioSN13 (SN13 K5-1B8) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections. Applications Tested: This eBioSN13 (SN13 K5-1B8) antibody has been tested by flow cytometric analysis of SN13 cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- eBioSN13 (SN13 K5-1B8)
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Influence of Mycobacterium bovis bacillus Calmette Guérin on in vitro induction of CD1 molecules in human adherent mononuclear cells.
Structure and expression of the human thymocyte antigens CD1a, CD1b, and CD1c.
Giuliani A, Prete SP, Graziani G, Aquino A, Balduzzi A, Sugita M, Brenner MB, Iona E, Fattorini L, Orefici G, Porcelli SA, Bonmassar E
Infection and immunity 2001 Dec;69(12):7461-70
Infection and immunity 2001 Dec;69(12):7461-70
Structure and expression of the human thymocyte antigens CD1a, CD1b, and CD1c.
Martin LH, Calabi F, Lefebvre FA, Bilsland CA, Milstein C
Proceedings of the National Academy of Sciences of the United States of America 1987 Dec;84(24):9189-93
Proceedings of the National Academy of Sciences of the United States of America 1987 Dec;84(24):9189-93
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of Molt-4 cell line with 0.5 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.5 µg of Anti-Human CD1b Purified (filled histogram) followed by Anti-Mouse IgG Biotin (Product # 13-4013-85) and Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL