Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 17-0018-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD1b Monoclonal Antibody (eBioSN13 (SN13 K5-1B8)), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioSN13 monoclonal antibody reacts with human CD1b. The CD1 molecules are similar to MHC class I, but rather than presenting peptide antigens to T-cell receptors (TCR), they present a diversity of lipid and glycolipid antigens which are derived from self as well as from mycobacteria which include sphingolipids, mycolic acid derivatives and phosphatidylinositols such as liparabinomannan (LAM) and glucose monomycolate (GMM). CD1b is 45 kDa, and like the other CD1 antigens, is expressed as a heterodimer composed of the CD1b noncovalently paired with beta2-microglobulin. Applications Reported: This eBioSN13 (SN13 K5-1B8) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioSN13 (SN13 K5-1B8) antibody has been pre-titrated and tested by flow cytometric analysis of the Molt-4 cell line. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- eBioSN13 (SN13 K5-1B8)
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Aryl Hydrocarbon Receptor Controls Monocyte Differentiation into Dendritic Cells versus Macrophages.
Human XCR1+ dendritic cells derived in vitro from CD34+ progenitors closely resemble blood dendritic cells, including their adjuvant responsiveness, contrary to monocyte-derived dendritic cells.
Influence of Mycobacterium bovis bacillus Calmette Guérin on in vitro induction of CD1 molecules in human adherent mononuclear cells.
CD1b restricts the response of human CD4-8- T lymphocytes to a microbial antigen.
Structure and expression of the human thymocyte antigens CD1a, CD1b, and CD1c.
Goudot C, Coillard A, Villani AC, Gueguen P, Cros A, Sarkizova S, Tang-Huau TL, Bohec M, Baulande S, Hacohen N, Amigorena S, Segura E
Immunity 2017 Sep 19;47(3):582-596.e6
Immunity 2017 Sep 19;47(3):582-596.e6
Human XCR1+ dendritic cells derived in vitro from CD34+ progenitors closely resemble blood dendritic cells, including their adjuvant responsiveness, contrary to monocyte-derived dendritic cells.
Balan S, Ollion V, Colletti N, Chelbi R, Montanana-Sanchis F, Liu H, Vu Manh TP, Sanchez C, Savoret J, Perrot I, Doffin AC, Fossum E, Bechlian D, Chabannon C, Bogen B, Asselin-Paturel C, Shaw M, Soos T, Caux C, Valladeau-Guilemond J, Dalod M
Journal of immunology (Baltimore, Md. : 1950) 2014 Aug 15;193(4):1622-35
Journal of immunology (Baltimore, Md. : 1950) 2014 Aug 15;193(4):1622-35
Influence of Mycobacterium bovis bacillus Calmette Guérin on in vitro induction of CD1 molecules in human adherent mononuclear cells.
Giuliani A, Prete SP, Graziani G, Aquino A, Balduzzi A, Sugita M, Brenner MB, Iona E, Fattorini L, Orefici G, Porcelli SA, Bonmassar E
Infection and immunity 2001 Dec;69(12):7461-70
Infection and immunity 2001 Dec;69(12):7461-70
CD1b restricts the response of human CD4-8- T lymphocytes to a microbial antigen.
Porcelli S, Morita CT, Brenner MB
Nature 1992 Dec 10;360(6404):593-7
Nature 1992 Dec 10;360(6404):593-7
Structure and expression of the human thymocyte antigens CD1a, CD1b, and CD1c.
Martin LH, Calabi F, Lefebvre FA, Bilsland CA, Milstein C
Proceedings of the National Academy of Sciences of the United States of America 1987 Dec;84(24):9189-93
Proceedings of the National Academy of Sciences of the United States of America 1987 Dec;84(24):9189-93
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of Molt-4 cells with Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD1b APC (purple histogram). Total viable cells were used for analysis.