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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Flow cytometry [3]
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- Product number
- MA1-4997 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FceR1 alpha Monoclonal Antibody (CRA1 (AER37))
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA1-4997 detects Fc epsilonR1 alpha from human samples. The CRA1 (AER37) monoclonal antibody reacts with the Fc epsilonR1 alpha subunit on a region that does not overlap the region of the IgE binding site, thus it does not compete with IgE for the receptor bindings. MA1-4997 has been successfully used in Western blot, immunocytochemistry, flow cytometry, and immunohistochemistry (paraffin, frozen) applications. The MA1-4997 immunogen is region of Fc epsilonR1 alpha which does not overlap with the IgE binding site. The epitope has been mapped to amino acids 26-110.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CRA1 (AER37)
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Carbonic anhydrase enzymes regulate mast cell-mediated inflammation.
NADPH oxidase-independent formation of extracellular DNA traps by basophils.
Henry EK, Sy CB, Inclan-Rico JM, Espinosa V, Ghanny SS, Dwyer DF, Soteropoulos P, Rivera A, Siracusa MC
The Journal of experimental medicine 2016 Aug 22;213(9):1663-73
The Journal of experimental medicine 2016 Aug 22;213(9):1663-73
NADPH oxidase-independent formation of extracellular DNA traps by basophils.
Morshed M, Hlushchuk R, Simon D, Walls AF, Obata-Ninomiya K, Karasuyama H, Djonov V, Eggel A, Kaufmann T, Simon HU, Yousefi S
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 1;192(11):5314-23
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 1;192(11):5314-23
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FceR1 alpha in TH-2 induced neutrophils using an FceR1 alpha monoclonal antibody (Product # MA1-4997). FceR1 alpha expression induced by TH-2 cytokines in neutrophils from allergic asthmatics. Total protein lysates were subjected to immunoprecipitation with IgE/anti IgE and Western blotting with (Product # MA1-4997). Basophilic cell line (KU812) was used as positive control. Negative control corresponds to neutrophil protein lysate analyzed without IgE/anti-IgE immunoprecipitation. (Image from Alphonse MP et al. PLoS One. 2008 Apr 2;3(4): e1921.)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow-cytometry of CHO/Fc epsilon R1 alpha cells with FceR1 alpha Monoclonal Antibody (Product # MA1-4997, CRA1) and FceR1 alpha Monoclonal Antibody (Product # MA1-4998, CRA2). CHO cells were transfected with plasmid expressing human Fc epsilon RI alpha.The second antibody is FITC-conjugated anti-mouse IgG2b antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow-cytometry of CHO/Fc epsilon R1 alpha cells with FceR1 alpha Monoclonal Antibody (Product # MA1-4997, CRA1) and FceR1 alpha Monoclonal Antibody (Product # MA1-4998, CRA2). CHO cells were transfected with plasmid expressing human Fc epsilon RI alpha.The second antibody is FITC-conjugated anti-mouse IgG2b antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow-cytometry of CHO/Fc epsilon R1 alpha cells with FceR1 alpha Monoclonal Antibody (Product # MA1-4997, CRA1) and FceR1 alpha Monoclonal Antibody (Product # MA1-4998, CRA2). CHO cells were transfected with plasmid expressing human Fc epsilon RI alpha.The second antibody is FITC-conjugated anti-mouse IgG2b antibody.
