H00002205-M01

antibody from Abnova Corporation

Targeting: FCER1A FCE1A

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002205-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002205-M01, RRID:AB_425428

Product name

FCER1A monoclonal antibody (M01), clone 2C12-3B6

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant FCER1A.

Antigen sequence

MAPAMESPTLLCVALLFFAPDGVLAVPQKPKVSLN
PPWNRIFKGENVTLTCNGNNFFEVSSTKWFHNGSL
SEETNSSLNIVNAKFEDSGEYKCQHQQVNESEPVY
LEVFSDWLLLQASAEVVMEGQPLFLRCHGWRNWDV
YKVIYYKDGEALKYWYENHNISITNATVEDSGTYY
CTGKVWQLDYESEPLNITVIKAPREKYWLQFFIPL
LVVILFAVDTGLFISTQQQVTFLLKIKRTRKGFRL
LNPHPKPNPKNN


Isotype

IgG

Antibody clone number

2C12-3B6

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

FCER1A monoclonal antibody (M01), clone 2C12-3B6 Western Blot analysis of FCER1A expression in A-549 ( Cat # L025V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of FCER1A expression in transfected 293T cell line by FCER1A monoclonal antibody (M01), clone 2C12-3B6.Lane 1: FCER1A transfected lysate(29.6 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged FCER1A is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol