Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 11-5899-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The AER-37 monoclonal antibody reacts with the Fc epsilon RI alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI apha is expressed on mast and basophil cells and is upregulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions. Applications Reported: This AER-37 (CRA1) antibody has been reported for use in flow cytometric analysis. Applications Tested: This AER-37 (CRA1) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- AER-37 (CRA1)
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Carbonic anhydrase enzymes regulate mast cell-mediated inflammation.
Expression of DP2 (CRTh2), a prostaglandin D₂ receptor, in human mast cells.
Carboplatin-induced severe hypersensitivity reaction: role of IgE-dependent basophil activation and FcεRI.
Critical Roles for PU.1, GATA1, and GATA2 in the expression of human FcεRI on mast cells: PU.1 and GATA1 transactivate FCER1A, and GATA2 transactivates FCER1A and MS4A2.
Expression of high-affinity IgE receptor on human peripheral blood dendritic cells in children.
IgE- and FcepsilonRI-mediated migration of human basophils.
IgE enhances Fc epsilon receptor I expression and IgE-dependent release of histamine and lipid mediators from human umbilical cord blood-derived mast cells: synergistic effect of IL-4 and IgE on human mast cell Fc epsilon receptor I expression and mediator release.
Henry EK, Sy CB, Inclan-Rico JM, Espinosa V, Ghanny SS, Dwyer DF, Soteropoulos P, Rivera A, Siracusa MC
The Journal of experimental medicine 2016 Aug 22;213(9):1663-73
The Journal of experimental medicine 2016 Aug 22;213(9):1663-73
Expression of DP2 (CRTh2), a prostaglandin D₂ receptor, in human mast cells.
Moon TC, Campos-Alberto E, Yoshimura T, Bredo G, Rieger AM, Puttagunta L, Barreda DR, Befus AD, Cameron L
PloS one 2014;9(9):e108595
PloS one 2014;9(9):e108595
Carboplatin-induced severe hypersensitivity reaction: role of IgE-dependent basophil activation and FcεRI.
Iwamoto T, Hirai H, Yamaguchi N, Kobayashi N, Sugimoto H, Tabata T, Okuda M
Cancer science 2014 Nov;105(11):1472-9
Cancer science 2014 Nov;105(11):1472-9
Critical Roles for PU.1, GATA1, and GATA2 in the expression of human FcεRI on mast cells: PU.1 and GATA1 transactivate FCER1A, and GATA2 transactivates FCER1A and MS4A2.
Inage E, Kasakura K, Yashiro T, Suzuki R, Baba Y, Nakano N, Hara M, Tanabe A, Oboki K, Matsumoto K, Saito H, Niyonsaba F, Ohtsuka Y, Ogawa H, Okumura K, Shimizu T, Nishiyama C
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 15;192(8):3936-46
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 15;192(8):3936-46
Expression of high-affinity IgE receptor on human peripheral blood dendritic cells in children.
Vasudev M, Cheung DS, Pincsak H, Li SH, Yan K, Simpson P, Dasu T, Grayson MH
PloS one 2012;7(2):e32556
PloS one 2012;7(2):e32556
IgE- and FcepsilonRI-mediated migration of human basophils.
Suzukawa M, Hirai K, Iikura M, Nagase H, Komiya A, Yoshimura-Uchiyama C, Yamada H, Ra C, Ohta K, Yamamoto K, Yamaguchi M
International immunology 2005 Sep;17(9):1249-55
International immunology 2005 Sep;17(9):1249-55
IgE enhances Fc epsilon receptor I expression and IgE-dependent release of histamine and lipid mediators from human umbilical cord blood-derived mast cells: synergistic effect of IL-4 and IgE on human mast cell Fc epsilon receptor I expression and mediator release.
Yamaguchi M, Sayama K, Yano K, Lantz CS, Noben-Trauth N, Ra C, Costa JJ, Galli SJ
Journal of immunology (Baltimore, Md. : 1950) 1999 May 1;162(9):5455-65
Journal of immunology (Baltimore, Md. : 1950) 1999 May 1;162(9):5455-65
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human IgE PE (Product # 12-6986-42) and Mouse IgG2b K Isotype Control FITC (Product # 11-4732-42) (left) or Anti-Human Fc epsilon Receptor I alpha (FceR1) FITC (right). Cells in the lymphocyte gate were used for analysis (note: basophils reside in FSC/SSC position of lymphocytes).
- Conjugate
- Green dye