Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 14-5899-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The AER-37 monoclonal antibody reacts with the FcεRIalpha subunit, an IgE-binding subunit lacking signal-transducing ability. FcεRIalpha is expressed on mast and basophil cells and is upregulated by the presence of IgE. FcεRIalpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The FcεRI complex plays an important role in triggering IgE-mediated allergic reactions. Applications Reported: The AER-37 (CRA1) antibody has been reported for use in flow cytometric analysis. It has been reported to not block IgE binding. Applications Tested: The AER-37 (CRA1) antibody has been tested by flow cytometric analysis of peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- AER-37 (CRA1)
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Clinical efficacy of omalizumab in chronic spontaneous urticaria is associated with a reduction of FcεRI-positive cells in the skin.
Human basophils are a source of - and are differentially activated by - IL-31.
Carbonic anhydrase enzymes regulate mast cell-mediated inflammation.
Mast cells form antibody-dependent degranulatory synapse for dedicated secretion and defence.
NADPH oxidase-independent formation of extracellular DNA traps by basophils.
Regulation of the human Fc epsilon RI alpha-chain distal promoter.
IgE enhances Fc epsilon receptor I expression and IgE-dependent release of histamine and lipid mediators from human umbilical cord blood-derived mast cells: synergistic effect of IL-4 and IgE on human mast cell Fc epsilon receptor I expression and mediator release.
Functional expression of the high affinity receptor for IgE (FcepsilonRI) in human platelets and its' intracellular expression in human megakaryocytes.
The alpha subunit of the human IgE receptor (FcERI) is sufficient for high affinity IgE binding.
Metz M, Staubach P, Bauer A, Brehler R, Gericke J, Kangas M, Ashton-Chess J, Jarvis P, Georgiou P, Canvin J, Hillenbrand R, Erpenbeck VJ, Maurer M
Theranostics 2017;7(5):1266-1276
Theranostics 2017;7(5):1266-1276
Human basophils are a source of - and are differentially activated by - IL-31.
Raap U, Gehring M, Kleiner S, Rüdrich U, Eiz-Vesper B, Haas H, Kapp A, Gibbs BF
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2017 Apr;47(4):499-508
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2017 Apr;47(4):499-508
Carbonic anhydrase enzymes regulate mast cell-mediated inflammation.
Henry EK, Sy CB, Inclan-Rico JM, Espinosa V, Ghanny SS, Dwyer DF, Soteropoulos P, Rivera A, Siracusa MC
The Journal of experimental medicine 2016 Aug 22;213(9):1663-73
The Journal of experimental medicine 2016 Aug 22;213(9):1663-73
Mast cells form antibody-dependent degranulatory synapse for dedicated secretion and defence.
Joulia R, Gaudenzio N, Rodrigues M, Lopez J, Blanchard N, Valitutti S, Espinosa E
Nature communications 2015 Jan 28;6:6174
Nature communications 2015 Jan 28;6:6174
NADPH oxidase-independent formation of extracellular DNA traps by basophils.
Morshed M, Hlushchuk R, Simon D, Walls AF, Obata-Ninomiya K, Karasuyama H, Djonov V, Eggel A, Kaufmann T, Simon HU, Yousefi S
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 1;192(11):5314-23
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 1;192(11):5314-23
Regulation of the human Fc epsilon RI alpha-chain distal promoter.
Hasegawa M, Nishiyama C, Nishiyama M, Akizawa Y, Takahashi K, Ito T, Furukawa S, Ra C, Okumura K, Ogawa H
Journal of immunology (Baltimore, Md. : 1950) 2003 Apr 1;170(7):3732-8
Journal of immunology (Baltimore, Md. : 1950) 2003 Apr 1;170(7):3732-8
IgE enhances Fc epsilon receptor I expression and IgE-dependent release of histamine and lipid mediators from human umbilical cord blood-derived mast cells: synergistic effect of IL-4 and IgE on human mast cell Fc epsilon receptor I expression and mediator release.
Yamaguchi M, Sayama K, Yano K, Lantz CS, Noben-Trauth N, Ra C, Costa JJ, Galli SJ
Journal of immunology (Baltimore, Md. : 1950) 1999 May 1;162(9):5455-65
Journal of immunology (Baltimore, Md. : 1950) 1999 May 1;162(9):5455-65
Functional expression of the high affinity receptor for IgE (FcepsilonRI) in human platelets and its' intracellular expression in human megakaryocytes.
Hasegawa S, Pawankar R, Suzuki K, Nakahata T, Furukawa S, Okumura K, Ra C
Blood 1999 Apr 15;93(8):2543-51
Blood 1999 Apr 15;93(8):2543-51
The alpha subunit of the human IgE receptor (FcERI) is sufficient for high affinity IgE binding.
Hakimi J, Seals C, Kondas JA, Pettine L, Danho W, Kochan J
The Journal of biological chemistry 1990 Dec 25;265(36):22079-81
The Journal of biological chemistry 1990 Dec 25;265(36):22079-81
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG2b kappa Isotype Control Purified (Product # 14-4732-82) (green histogram) or Anti-Human Fc epsilon Receptor I a (FcepsilonR1) Purified (blue histogram) followed by Anti-Mouse IgG Biotin (Product # 13-4013-85) and Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.