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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 14-5899-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The AER-37 monoclonal antibody reacts with the FcεRIalpha subunit, an IgE-binding subunit lacking signal-transducing ability. FcεRIalpha is expressed on mast and basophil cells and is upregulated by the presence of IgE. FcεRIalpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The FcεRI complex plays an important role in triggering IgE-mediated allergic reactions.
- Antibody clone number
- AER-37 (CRA1)
- Concentration
- 0.5 mg/mL
Submitted references Clinical efficacy of omalizumab in chronic spontaneous urticaria is associated with a reduction of FcεRI-positive cells in the skin.
Human basophils are a source of - and are differentially activated by - IL-31.
Carbonic anhydrase enzymes regulate mast cell-mediated inflammation.
Mast cells form antibody-dependent degranulatory synapse for dedicated secretion and defence.
NADPH oxidase-independent formation of extracellular DNA traps by basophils.
Redefinition of the human mast cell transcriptome by deep-CAGE sequencing.
Interferons modulate Fc epsilon RI-dependent production of autoregulatory IL-10 by circulating human monocytoid dendritic cells.
Regulation of the human Fc epsilon RI alpha-chain distal promoter.
IgE enhances Fc epsilon receptor I expression and IgE-dependent release of histamine and lipid mediators from human umbilical cord blood-derived mast cells: synergistic effect of IL-4 and IgE on human mast cell Fc epsilon receptor I expression and mediator release.
Functional expression of the high affinity receptor for IgE (FcepsilonRI) in human platelets and its' intracellular expression in human megakaryocytes.
The alpha subunit of the human IgE receptor (FcERI) is sufficient for high affinity IgE binding.
Metz M, Staubach P, Bauer A, Brehler R, Gericke J, Kangas M, Ashton-Chess J, Jarvis P, Georgiou P, Canvin J, Hillenbrand R, Erpenbeck VJ, Maurer M
Theranostics 2017;7(5):1266-1276
Theranostics 2017;7(5):1266-1276
Human basophils are a source of - and are differentially activated by - IL-31.
Raap U, Gehring M, Kleiner S, Rüdrich U, Eiz-Vesper B, Haas H, Kapp A, Gibbs BF
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2017 Apr;47(4):499-508
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2017 Apr;47(4):499-508
Carbonic anhydrase enzymes regulate mast cell-mediated inflammation.
Henry EK, Sy CB, Inclan-Rico JM, Espinosa V, Ghanny SS, Dwyer DF, Soteropoulos P, Rivera A, Siracusa MC
The Journal of experimental medicine 2016 Aug 22;213(9):1663-73
The Journal of experimental medicine 2016 Aug 22;213(9):1663-73
Mast cells form antibody-dependent degranulatory synapse for dedicated secretion and defence.
Joulia R, Gaudenzio N, Rodrigues M, Lopez J, Blanchard N, Valitutti S, Espinosa E
Nature communications 2015 Jan 28;6:6174
Nature communications 2015 Jan 28;6:6174
NADPH oxidase-independent formation of extracellular DNA traps by basophils.
Morshed M, Hlushchuk R, Simon D, Walls AF, Obata-Ninomiya K, Karasuyama H, Djonov V, Eggel A, Kaufmann T, Simon HU, Yousefi S
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 1;192(11):5314-23
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 1;192(11):5314-23
Redefinition of the human mast cell transcriptome by deep-CAGE sequencing.
Motakis E, Guhl S, Ishizu Y, Itoh M, Kawaji H, de Hoon M, Lassmann T, Carninci P, Hayashizaki Y, Zuberbier T, Forrest AR, Babina M, FANTOM consortium
Blood 2014 Apr 24;123(17):e58-67
Blood 2014 Apr 24;123(17):e58-67
Interferons modulate Fc epsilon RI-dependent production of autoregulatory IL-10 by circulating human monocytoid dendritic cells.
Le T, Tversky J, Chichester KL, Bieneman AP, Huang SK, Wood RA, Schroeder JT
The Journal of allergy and clinical immunology 2009 Jan;123(1):217-23
The Journal of allergy and clinical immunology 2009 Jan;123(1):217-23
Regulation of the human Fc epsilon RI alpha-chain distal promoter.
Hasegawa M, Nishiyama C, Nishiyama M, Akizawa Y, Takahashi K, Ito T, Furukawa S, Ra C, Okumura K, Ogawa H
Journal of immunology (Baltimore, Md. : 1950) 2003 Apr 1;170(7):3732-8
Journal of immunology (Baltimore, Md. : 1950) 2003 Apr 1;170(7):3732-8
IgE enhances Fc epsilon receptor I expression and IgE-dependent release of histamine and lipid mediators from human umbilical cord blood-derived mast cells: synergistic effect of IL-4 and IgE on human mast cell Fc epsilon receptor I expression and mediator release.
Yamaguchi M, Sayama K, Yano K, Lantz CS, Noben-Trauth N, Ra C, Costa JJ, Galli SJ
Journal of immunology (Baltimore, Md. : 1950) 1999 May 1;162(9):5455-65
Journal of immunology (Baltimore, Md. : 1950) 1999 May 1;162(9):5455-65
Functional expression of the high affinity receptor for IgE (FcepsilonRI) in human platelets and its' intracellular expression in human megakaryocytes.
Hasegawa S, Pawankar R, Suzuki K, Nakahata T, Furukawa S, Okumura K, Ra C
Blood 1999 Apr 15;93(8):2543-51
Blood 1999 Apr 15;93(8):2543-51
The alpha subunit of the human IgE receptor (FcERI) is sufficient for high affinity IgE binding.
Hakimi J, Seals C, Kondas JA, Pettine L, Danho W, Kochan J
The Journal of biological chemistry 1990 Dec 25;265(36):22079-81
The Journal of biological chemistry 1990 Dec 25;265(36):22079-81
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG2b kappa Isotype Control Purified (Product # 14-4732-82) (green histogram) or Anti-Human Fc epsilon Receptor I a (FcepsilonR1) Purified (blue histogram) followed by Anti-Mouse IgG Biotin (Product # 13-4013-85) and Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.
